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Modularly assembled multiplex prime editors for simultaneous editing of agronomically important genes in rice.
Gupta A, Liu B, Raza S, Chen QJ, Yang B
Plant Commun. 2023 Oct 26:100741. doi: 10.1016/j.xplc.2023.100741.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
213049pG3H-PE3max-attR1R2Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites
213050pAG79-attL1-pegRNA-attL2Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attL2
213051pCR8-attL1-pegRNA-attR3Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR3
213052pCR8-attL3-pegRNA-attL2Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2
213053pCR8-attL1-pegRNA-attR5Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5
213054pCR8-attL5-pegRNA-attR3Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3
213055pCR8-attL5-pegRNA-attL4Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4
213056pCR8-attR4-pegRNA-attR3Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3

Antibodies from Article