A versatile CRISPR-Cas13d platform for multiplexed transcriptomic regulation and metabolic engineering in primary human T cells
Tieu V, Sotillo E, Bjelajac JR, Chen C, Malipatlolla M, Guerrero JA, Xu P, Quinn PJ, Fisher C, Klysz D, Mackall CL, Qi LS
Cell. 2024 Feb 21. doi: 10.1016/j.cell.2024.01.035 Article
Tieu V, Sotillo E, Bjelajac JR, Chen C, Malipatlolla M, Guerrero JA, Xu P, Quinn PJ, Fisher C, Klysz D, Mackall CL, Qi LS
Cell. 2024 Feb 21. doi: 10.1016/j.cell.2024.01.035 Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214875 | pSLQ4419 pHR (hU6-BsmBI-BsmBI-EFS-PuroR-WPRE) | Empty lentiviral backbone for guide array cloning |
| 214876 | pSLQ7501 pHR (hU6-crNT-EFS-PuroR-WPRE) | Lentiviral vector encoding RfxCas13d nontargeting control guide |
| 214877 | pSLQ7529 pHR (hU6-crLPT-EFS-PuroR-WPRE) | Lentiviral vector encoding RfxCas13d targeting LPT guide array |
| 214878 | pSLQ7534 pHR (hU6-crSURF1-EFS-PuroR-WPRE) | Lentiviral vector encoding RfxCas13d targeting SURF1 guide array |
| 214879 | pSLQ7535 pHR (hU6-crSURF2-EFS-PuroR-WPRE) | Lentiviral vector encoding RfxCas13d targeting SURF2 guide array |
| 214880 | pSLQ7556 pHR (hU6-crCD46-EFS-PuroR-WPRE) | Lentiviral vector encoding RfxCas13d targeting CD46 guide |
| 214881 | pSLQ7558 pHR (hU6-crB2M-EFS-PuroR-WPRE) | Lentiviral vector encoding RfxCas13d targeting B2M guide |
| 214882 | pSLQ7578 pHR (hU6-crPROX-EFS-PuroR-WPRE) | Lentiviral vector encoding RfxCas13d targeting PROX guide array |
| 214883 | pSLQ7820 pHR (hU6-crPURI-EFS-PuroR-WPRE) | Lentiviral vector encoding RfxCas13d targeting PURI guide array |
| 214884 | pSLQ7854 pHR (hU6-crGLY-EFS-PuroR-WPRE) | Lentiviral vector encoding RfxCas13d targeting GLY guide array |
| 214885 | pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE) | Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion |