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Addgene
A selection of Golden Gate vectors to simplify recombinant protein production in Escherichia coli
Fairhead M, Koekemoer L, Williams E, von Delft F
bioRxiv 2024.02.13.579886 Article

Plasmids from Article

ID Plasmid Purpose
215789pNIC-NHSTIIT-GGCloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage
215790pNIC-OmpA-NHSTIIT-GGCloning of target gene with N terminal OmpA SEC pathway signal peptide His6 Twin Strep tag and TEV cleavage
215791pNIC-SufI-NHSTIIT-GGCloning of target gene with N terminal SufI TatA pathway signal peptide His6 Twin Strep tag and TEV cleavage
215792pNIC-TolB-NHSTIIT-GGCloning of target gene with N terminal TolB SRP pathway signal peptide N terminal His6 Twin Strep tag and TEV cleavage
215793pNIC-PelB-NH10T-AVITAG-GGCloning of target gene with N terminal PelB SEC pathway signal peptide His10 and AVITAG and TEV cleavage
215794pNIC-MBP-TEV-GGCloning of target gene with N terminal MBP tag and TEV cleavage
215795pNIC-GST-TEV-GGCloning of target gene with N terminal GST tag and TEV cleavage
215796pNIC-HIS6-THRX-TEV-GGCloning of target gene with N terminal His6 Thioredoxin tag and TEV cleavage
215797pNIC-HIS10-ZBASIC-TEV-GGCloning of target gene with N-terminal His10 Zbasic tag and TEV cleavage
215798pNIC-HIS6-NUSA-TEV-GGCloning of target gene with N terminal His6 NUSA tag and TEV cleavage
215799pNIC-HIS6-SUMO1-GGCloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage
215800pNIC-HIS6-MBP-TEV-GGCloning of target gene with N terminal His6 MBP tag and TEV Cleavage
215801pNIC-HIS6-GST-TEV-GGCloning of target gene with N terminal His6 GST tag and TEV cleavage
215802pNIC-NHSTIIT-AVITAG-GGCloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage non cleavable N-terminal AVITAG
215803pNIC-CT-sfGFP-HN-GGCloning of target gene with C terminal TEV cleavable sfGFP HisAsn6 tag
215804pNIC-CTH10STII-GGCloning of target gene with C terminal TEV cleavage of His10 and Twin Strep tag
215805pNIC- AVITAG-CTH10STII- -GGCloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tag
215806pNIC-NH10-GGCloning of target gene with N terminal non cleavable His10 tag
215807pNIC-CH10-GGCloning of target gene with C terminal non cleavable His10 tag
215808pNIC-HIS6-SUMO1-AVITAG-GGCloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage non cleavable N-terminal avitag
215809pNIC-HIS6-GST-TEV-AVITAG-GGCloning of target gene with N terminal His6 GST tag and TEV cleavage non cleavable N-terminal avitag
215810pNIC-NoTag-GGCloning of target gene with no tags

Antibodies from Article