Computationally designed sensors detect endogenous Ras activity and signaling effectors at subcellular resolution.
Zhang JZ, Nguyen WH, Greenwood N, Rose JC, Ong SE, Maly DJ, Baker D
Nat Biotechnol. 2024 Jan 25. doi: 10.1038/s41587-023-02107-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216496 | pcDNA3 Ras-LOCKR-S | FRET-based reporter for Ras activity, untargeted |
| 216497 | pcDNA3 Ras-LOCKR-S R89L (negative control) | FRET-based reporter for Ras activity, untargeted, negative control |
| 216498 | pcDNA3 PM-Ras-LOCKR-S | FRET-based reporter for Ras activity, plasma membrane localized |
| 216499 | pcDNA3 Golgi-Ras-LOCKR-S | FRET-based reporter for Ras activity, Golgi localized |
| 216500 | pcDNA3 ER-Ras-LOCKR-S | FRET-based reporter for Ras activity, ER localized |
| 216501 | pcDNA3 Mito-Ras-LOCKR-S | FRET-based reporter for Ras activity, mitochondria localized |
| 216502 | pcDNA3 Ras-LOCKR-PL Cage | Ras activity dependent proximity labeler, Cage only, untargeted |
| 216503 | pcDNA3 Ras-LOCKR-PL Key | Ras activity dependent proximity labeler, Key only, untargeted |
| 216504 | pcDNA3 PM-Ras-LOCKR-PL Key | Ras activity dependent proximity labeler, Key only, plasma membrane localized |
| 216505 | pcDNA3 Golgi-Ras-LOCKR-PL Key | Ras activity dependent proximity labeler, Key only, Golgi localized |
| 216506 | pcDNA3 ER-Ras-LOCKR-PL Key | Ras activity dependent proximity labeler, Key only, ER localized |
| 216507 | pcDNA3 Mito-Ras-LOCKR-PL Key | Ras activity dependent proximity labeler, Key only, mitochondria localized |