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Peter Cockerill Lab: Development of receptor and oncogene-responsive luciferase reporter vectors that are activated via kinase signalling to AP-1.

Danial J L Coleman, Luke Ames, Hurooul Aain, Joanna Gamble, Kinga Koscielniak, Zijin Zhang, Cherisse Lau, Abigail Joyce, and Peter N Cockerill.
Experimental Hematology, 2026 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
71258pAP1-3luciferase reporter with 3 copies of the stromelylisin gene AP-1 site in front of hGM-CSF -55 to +28 minimal promoter
217413LEGO-GM55Luciferase expression vector with the minimal GM-CSF promoter
217414LEGO-AP1x3-GM55Luciferase expression vector with 3 AP-1 sites and the minimal GM-CSF promoter. Alias: LEGO-GM55-AP-1x3
217415LEGO-AP1x6-GM55Luciferase expression vector with 6 AP-1 sites and the minimal GM-CSF promoter. Alias: LEGO-GM55-AP-1x6
217416LEGO-pDHS-AP1x6-GM55Luciferase expression vector with 6 AP-1 sites and the minimal GM-CSF promoter, and a chromatin priming element. Alias: LEGO-GM55-AP-1x6-pDHS(IL-3)
217418LEGO-pDHS-AP1x6-DUSP5Luciferase expression vector with 6 AP-1 sites and the full length DUSP5 promoter, and a chromatin priming element. Alias: LEGO-DUSP5p-AP-1x6
217419LEGO-pDHS-ITDE-AP1x6-DUSP5Luciferase expression vector with an oncogene-inducible enhancer, 6 AP-1 sites, the full length DUSP5 promoter, and a chromatin priming element. Alias: LEGO-DUSP5p-AP-1x6-ITDe

Antibodies from Article