Delivering a multi-color, cell-based CRISPR experience to low-resource classrooms.
Walters AJ, Lyons AT, Williams AA, Porter D, Riddle C, Moravek A, Lunt M, van Renselaar J, Hall D, Barnes T, Scribner A, Bryson C, Sims R, Miller C, Jackson RN
J Microbiol Biol Educ. 2026 Apr 30;27(1):e0023225. doi: 10.1128/jmbe.00232-25. Epub 2026 Mar 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220922 | pCas9_antiBFP | Expresses Cas9 with a BFP targeting crRNA for use in targeting the BFP plasmid in a cell based assay |
| 220923 | pCas9_antiGFP | Expresses Cas9 with a GFP targeting crRNA for use in targeting the GFP plasmid in a cell based assay |
| 220924 | pCas9_antiOFP | Expresses Cas9 with a OFP targeting crRNA for use in targeting the OFP plasmid in a cell based assay |
| 220932 | pYTK001_GFP | Expresses GFP in bacterial cells |
| 220933 | pYTK001_BFP | Expresses BFP in bacterial cells |
| 220934 | pSB1A3_OFP | Expresses OFP in bacterial cells |