A novel expression system for imaging single-molecule fluorescence in Haloferax volcanii WR806 enables visualization of altered Cas1 dynamics during UV-induced DNA damage response.
Schrage PR, Afonina U, Wortz J, Marchfelder A, Martens KJA, Saenz JP, Endesfelder U
Microlife. 2026 Apr 13;7:uqag014. doi: 10.1093/femsml/uqag014. eCollection 2026.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234669 | pUE001-Dendra2Hfx | encodes Dendra2Hfx in Haloferax volcanii under the tryptophan inducible p.tna promoter, trpA, ampR, shuttle vector for E.coli |
| 234670 | pUE001-Cas1:Dendra2Hfx | encodes Cas1:Dendra2Hfx fusion in Haloferax volcanii under the tryptophan inducible p.tna promoter, trpA, ampR, shuttle vector for E.coli |
| 234671 | pUE001-FtsZ1:Dendra2Hfx | encodes FtsZ1:Dendra2Hfx fusion in Haloferax volcanii under the tryptophan inducible p.tna promoter, trpA, ampR, shuttle vector for E.coli |