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Vacuum and Sonication Treatment Enable Efficient Transient Gene Expression in Various Monocot and Eudicot Plant Seedlings.

Li E, Geng Y, Khristoforova TR, Wang Y, Jones JW, Muchenje KT, Grillo E, Zhang H, Schmitz RJ, Demirer GS
ACS Synth Biol. 2026 Apr 17;15(4):1452-1466. doi: 10.1021/acssynbio.5c00855. Epub 2026 Mar 22. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
236636pYG18The upstream component of a geminiviral replicon
236637pYG29YPET transcription unit
236638pYG13The downstream component of a geminiviral replicon
236639pYG34YPET expression construct built based on pYG13, pYG18 and pYG29
236640pQQ7Transcriptional unit Fast-Red
236641pQQ128Transcriptional unit NanoLuc luciferase with Nopaline Synthase promoter and terminator, reverse orientation
236642pQQ132Transcriptional unit Green Enhanced Nano-lantern with Nitrate-Regulated Promoter and Arabidopsis heat shock protein 18.2 terminator
236643pQQ137NRP nitrate sensor built based on pQQ7, pQQ128, and pQQ132

Antibodies from Article