Proteome-scale tagging and functional screening in mammalian cells by ORFtag.
Nemcko F, Himmelsbach M, Loubiere V, Yelagandula R, Pagani M, Fasching N, Brennecke J, Elling U, Stark A, Ameres SL
Nat Methods. 2024 Sep;21(9):1668-1673. doi: 10.1038/s41592-024-02339-x. Epub 2024 Jul 5.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220981 | ORFtag_TetR-SD_Frame1 | ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame1 |
| 220982 | ORFtag_TetR-SD_Frame2 | ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame2 |
| 220983 | ORFtag_TetR-SD_Frame3 | ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame3 |
| 220984 | ORFtag_LambdaN-SD_Frame1 | ORFtag viral retroviral vector containing a constitutively active promoter and a LambdaN tag followed by a splice donor sequence in Frame1 |
| 220985 | ORFtag_LambdaN-SD_Frame2 | ORFtag viral retroviral vector containing a constitutively active promoter and a LambdaN tag followed by a splice donor sequence in Frame2 |
| 220986 | ORFtag_LambdaN-SD_Frame3 | ORFtag viral retroviral vector containing a constitutively active promoter and a LambdaN tag followed by a splice donor sequence in Frame3 |