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Barcoded overexpression screens in gut Bacteroidales identify genes with roles in carbon utilization and stress resistance.

Huang YY, Price MN, Hung A, Gal-Oz O, Tripathi S, Smith CW, Ho D, Carion H, Deutschbauer AM, Arkin AP
Nat Commun. 2024 Aug 5;15(1):6618. doi: 10.1038/s41467-024-50124-3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
211910pNBU2_repA1Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.
211911pNBU2_repA2Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.
211912pNBU2_repA3Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.

Antibodies from Article