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Design and evolution of the tetracycline repressor into sulfonylurea herbicide-responsive gene switches for field crops.

McBride KE, Kakani NK, Bozhanova NG, Fang J, Lowe K, Leija C, McGonigle B, Schreiter ER, Hermanson F, Chan M, Arling M, Wang N, Cho HJ, Richey C, Hoerster G, Marvin JS, Lenderts B, McReynolds MR, Hastings C, Madrigal A, Falco SC, Lassner MW, Anand A, Gordon-Kamm W, Looger LL
Nat Commun. 2026 Jun 8. doi: 10.1038/s41467-026-73848-w. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
242345pRSET-His-wtTetRBacterial expression of N-terminal 6xHis-tagged wtTetR
242346pRSET-His-L12-11Bacterial expression of N-terminal 6xHis-tagged L12-11
242347pRSET-His-L13-9Bacterial expression of N-terminal 6xHis-tagged L13-9
242348pRSET-His-L13-23Bacterial expression of N-terminal 6xHis-tagged L13-23
242349pRSET-His-L15-20Bacterial expression of N-terminal 6xHis-tagged L15-20
242350pRSET-His-CsL4.2-15Bacterial expression of N-terminal 6xHis-tagged CsL4.2-15
242351pRSET-His-CsL4.2-20Bacterial expression of N-terminal 6xHis-tagged CsL4.2-20
242352pcDNA6-L13-9Mammalian expression of L13-9
242353pcDNA6-L15-20Mammalian expression of L15-20
242354pcDNA6-CsL4.2-15Mammalian expression of CsL4.2-15
242355pcDNA6-CsL4.2-20Mammalian expression of CsL4.2-20
242356pcDNA4-sfGFPInducible mammalian expression of sfGFP

Antibodies from Article