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RHOBTB3 promotes proteasomal degradation of HIFalpha through facilitating hydroxylation and suppresses the Warburg effect.

Zhang CS, Liu Q, Li M, Lin SY, Peng Y, Wu D, Li TY, Fu Q, Jia W, Wang X, Ma T, Zong Y, Cui J, Pu C, Lian G, Guo H, Ye Z, Lin SC
Cell Res. 2015 Sep;25(9):1025-42. doi: 10.1038/cr.2015.90. Epub 2015 Jul 28. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
246202pBOBI N-HA hVHLUsed for transfection of cells for expression N-HA hVHL
246203pBOBI N-FLAG hVHLUsed for transfection of cells for expression N-FLAG hVHL
246204pcDNA 3.3 N-FLAG VHLUsed for transfection of cells for expression N-FLAG VHL
246205pBOBI N-FLAG LIMD1Used for transfection of cells for expression N-FLAG LIMD1
246206pBOBI N-HA LIMD1Used for transfection of cells for expression N-HA LIMD1
246207pcDNA 3.3 N-HA RhoBTB3Used for transfection of cells for expression N-HA RhoBTB3
246208pcDNA 3.3 N-MYC RhoBTB3Used for transfection of cells for expression N-MYC RhoBTB3
246209pBOBI N-HA EGLN1Used for transfection of cells for expression N-HA EGLN1
246210pBOBI C-FLAG EGLN1Used for transfection of cells for expression C-FLAG EGLN1
246211pBOBI N-HA HIF2aUsed for transfection of cells for expression N-HA HIF2a
246212pBOBI N-FLAG HIF2aUsed for transfection of cells for expression N-FLAG HIF2a
246213pBOBI no tag hHIF1aUsed for transfection of cells for expression hHIF1a
246214pcDNA 3.3 N-HA HIF1aUsed for transfection of cells for expression N-HA HIF1a
246216CMV5 N-HA hHIF1a P402AUsed for transfection of cells for expression N-HA hHIF1a P402A
246219pcDNA 3.3 N-FLAG hARNTUsed for transfection of cells for expression N-FLAG hARNT

Antibodies from Article