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Multiplexed, precise genome engineering in monocots with twin prime editing systems.

Li H, Chai Z, Shi X, Sun C, Zhang R, Zhang Q, Li Z, Zhang K, Lei Y, Gao C
Nat Biotechnol. 2026 Jun 5. doi: 10.1038/s41587-026-03174-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
246791p35C-epegRNA-ccdBExpress pegRNA in plant cells (protoplast or callus) for prime editing
246792p35C-gRNA-ccdBExpress gRNA in plant cells (protoplast or callus) for Cas9-mediated editing
246794pH35C-gRNA-Cas9Express Cas9 protein and gRNA in plant cells (protoplast or callus) for Cas9-mediated editing
246795pH35C-epegRNA-ePPEplusExpress ePPEplus and pegRNA in plant cells (protoplast or callus) for prime editing

Antibodies from Article