Expanding genetic engineering capabilities in Vibrio natriegens with the Vnat Collection.
Faber A, Politan RJ, Stukenberg D, Morris KM, Kim R, Jeon E, Inckemann R, Becker A, Thuronyi B, Fritz G
Nucleic Acids Res. 2025 Jul 8;53(13):gkaf580. doi: 10.1093/nar/gkaf580.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249835 | pVC_V_01_Part_Entry_sfGFP | Entry vector, standard, lv0, sfGFP |
| 249836 | pVC_V_02_Part_Entry_RFP | Entry vector, standard, lv0, mScarlet |
| 249838 | pVC_V_09_Res_Entry_mScarlet-I | Entry vector, ABR, lv0, mScarlet |
| 249839 | pVC0_1_02_5C1CLN | 5' connector, lv0, 5C1CLN |
| 249840 | pVC0_1_03_5C1RLN | 5' connector, lv0, 5C1CLN |
| 249841 | pVC0_1_04_5C1CSN | 5' connector, lv0, 5C1RLN |
| 249842 | pVC0_1_05_5C1RSN | 5' connector, lv0, 5C1CSN |
| 249843 | pVC0_1_06_5C1SI | 5' connector, lv0, 5C1RSN |
| 249844 | pVC0_1_07_5C2LN | 5' connector, lv0, 5C1SI |
| 249845 | pVC0_1_08_5C2SN | 5' connector, lv0,5C2LN |
| 249846 | pVC0_1_09_5C2SI | 5' connector, lv0, 5C2SI |
| 249847 | pVC0_1_10_5C3LN | 5' connector, lv0,5C3LN |
| 249848 | pVC0_1_11_5C3SN | 5' connector, lv0,5C3SN |
| 249850 | pVC0_1_13_5C4LN | 5' connector, lv0, 5C4LN |
| 249851 | pVC0_1_14_5C4SN | 5' connector, lv0, 5C4SN |
| 249852 | pVC0_1_15_5C4SI | 5' connector, lv0, 5C4SI |
| 249853 | pVC0_1_16_5C5LN | 5' connector, lv0, 5C5LN |
| 249854 | pVC0_1_17_5C5SN | 5' connector, lv0, 5C5SN |
| 249855 | pVC0_1_18_5C5CSI | 5' connector, lv0, 5C5CSI |
| 249857 | pVC0_1_20_5C0SN | 5' connector, lv0, 5C0SN |
| 249858 | pVC0_1-2_01_5C1_operon | 5' operon connector, lv0, 5C1 |
| 249859 | pVC0_1-2_02_5C2_operon | 5' operon connector, lv0, 5C2 |
| 249860 | pVC0_1-2_03_5C3_operon | 5' operon connector, lv0, 5C3 |
| 249861 | pVC0_1-2_04_5C4_operon | 5' operon connector, lv0, 5C4 |
| 249862 | pVC0_1-2_05_5C5_operon | 5' operon connector, lv0, 5C5 |
| 249863 | pVC0_2_02_PJ23100 | constitutive promoter, lv0, 6th strongest |
| 249864 | pVC0_2_03_PJ23101 | constitutive promoter, lv0, 4th strongest |
| 249865 | pVC0_2_04_PJ23102 | constitutive promoter, lv0, 5th strongest |
| 249866 | pVC0_2_05_PJ23103 | constitutive promoter, lv0, 20th strongest |
| 249867 | pVC0_2_06_PJ23104 | constitutive promoter, lv0, 2nd strongest |
| 249868 | pVC0_2_07_PJ23105 | constitutive promoter, lv0, 13th strongest |
| 249869 | pVC0_2_08_PJ23106 | constitutive promoter, lv0, 11th strongest |
| 249870 | pVC0_2_09_PJ23107 | constitutive promoter, lv0, 12th strongest |
| 249871 | pVC0_2_10_PJ23108 | constitutive promoter, lv0, 10th strongest |
| 249872 | pVC0_2_11_PJ23109 | constitutive promoter, lv0, 19th strongest |
| 249873 | pVC0_2_12_PJ23110 | constitutive promoter, lv0, 9th strongest |
| 249874 | pVC0_2_13_PJ23111 | constitutive promoter, lv0, strongest |
| 249875 | pVC0_2_14_PJ23113 | constitutive promoter, lv0, 18th strongest |
| 249876 | pVC0_2_15_PJ23114 | constitutive promoter, lv0, 16th strongest |
| 249877 | pVC0_2_16_PJ23115 | constitutive promoter, lv0, 14th strongest |
| 249878 | pVC0_2_17_PJ23116 | constitutive promoter, lv0, 15th strongest |
| 249879 | pVC0_2_18_PJ23117 | constitutive promoter, lv0, 17th strongest |
| 249880 | pVC0_2_19_PJ23118 | constitutive promoter, lv0, 8th strongest |
| 249881 | pVC0_2_20_PJ23119 | constitutive promoter, lv0, 3rd strongest |
| 249882 | pVC0_2_21_PDummy | constitutive promoter, lv0, dummy |
| 249884 | pVC0_2_23_PTet | inducible promoter, lv0, PTet_MC |
| 249885 | pVC0_2_29_PBDA (Vn) | inducible promoter, lv0, PBDA |
| 249886 | pVC0_2_33_PRham (Vn) | inducible promoter, lv0, PRham |
| 249887 | pVC0_2_39_PTtg | inducible promoter, lv0, PTtg |
| 249888 | pVC0_2_45_CinR_+1 | inducible promoter, lv0, PCin+1 |
| 249889 | pVC0_2_41_PPhlF | inducible promoter, lv0, PPhlF |
| 249890 | pVC0_2_42_PTet* | inducible promoter, lv0, PTet* |
| 249891 | pVC0_2_43_PTac | inducible promoter, lv0, PTac |
| 249893 | pVC0_2_50_P3B5B | inducible promoter, lv0, P3B5B |
| 249894 | pVC0_3_02_RB0029 | RBS, lv0, 7th strongest |
| 249895 | pVC0_3_03_RB0030 | RBS, lv0, strongest |
| 249896 | pVC0_3_04_RB0031 | RBS, lv0, 8th strongest |
| 249897 | pVC0_3_05_RB0032 | RBS, lv0, 5th strongest |
| 249898 | pVC0_3_06_RB0033 | RBS, lv0, 6th strongest |
| 249899 | pVC0_3_07_RB0034 | RBS, lv0, 3rd strongest |
| 249900 | pVC0_3_08_RB0035 | RBS, lv0, 2nd strongest |
| 249901 | pVC0_3_09_RB0064 | RBS, lv0, 4th strongest |
| 249902 | pVC0_3_10_Rdummy | RBS, lv0, dummy |
| 249903 | pVC0_4a_04_N3xFLAG | N-terminal tag, lv0, 3xFLAG |
| 249904 | pVC0_4a_05_N6xHis | N-terminal tag, lv0, 6xHis |
| 249905 | pVC0_4a_06_NAzurite (Vn) | N-terminal tag, lv0, Azurite |
| 249906 | pVC0_4a_07_NmTurquoise(Vn) | N-terminal tag, lv0, mTurquoise |
| 249907 | pVC0_4a_09_NmVenus (Vn) | N-terminal tag, lv0, mVenus |
| 249908 | pVC0_4a_10_NmScarlet-I (Vn) | N-terminal tag, lv0, mScarlet-I |
| 249909 | pVC0_4a_11_NmCherry (Vn) | N-terminal tag, lv0, mCherry |
| 249910 | pVC0_4a_12_NmKate (Vn) | N-terminal tag, lv0, mKate |
| 249911 | pVC0_4a_13_Nsfgfp(Vn) | N-terminal tag, lv0, sfGfp |
| 249912 | pVC0_4_02_CDSlux Operon | CDS, lv 0, lux operon |
| 249913 | pVC0_4_09_CDSAzurite (Vn) | CDS, lv 0, Azurite |
| 249914 | pVC0_4_10_CDSmTurquoise (Vn) | CDS, lv 0, mTurquoise |
| 249915 | pVC0_4_11_CDSmVenus (Vn) | CDS, lv 0, mVenus |
| 249916 | pVC0_4_12_CDSmScarlet-I (Vn) | CDS, lv 0, mScarlet-I |
| 249917 | pVC0_4_13_CDSmCherry (Vn) | CDS, lv 0, mCherry |
| 249918 | pVC0_4_14_CDSmKate-2 (Vn) | CDS, lv 0, mKate-2 |
| 249919 | pVC0_4_16_CDSVioA | CDS, lv 0, VioA |
| 249920 | pVC0_4_17_CDSVioB | CDS, lv 0, VioB |
| 249921 | pVC0_4_18_CDSVioC | CDS, lv 0, VioC |
| 249922 | pVC0_4_20_CDSVioE | CDS, lv 0, VioE |
| 249923 | pVC0_4_24_CDSNLuc | CDS, lv 0, NLuc |
| 249924 | pVC0_4_23_CDSsfgfp (Vn) | CDS, lv 0, sfGfp |
| 249925 | pVC0_5_02_TB0010 | terminator, pos 5, lv0, 2nd strongest |
| 249926 | pVC0_5_03_TB0015 | terminator, pos 5, lv0, strongest |
| 249927 | pVC0_5_04_TB1002 | terminator, pos 5, lv0, 4th strongest |
| 249928 | pVC0_5_05_TB1003 | terminator, pos 5, lv0, 5th strongest |
| 249929 | pVC0_5_06_TB1004 | terminator, pos 5, lv0, 9th strongest |
| 249930 | pVC0_5_07_TB1005 | terminator, pos 5, lv0, 8th strongest |
| 249931 | pVC0_5_08_TB1006 | terminator, pos 5, lv0, 3rd strongest |
| 249932 | pVC0_5_09_TB1007 | terminator, pos 5, lv0, 6th strongest |
| 249933 | pVC0_5_11_TB1009 | terminator, pos 5, lv0, 10th strongest |
| 249934 | pVC0_5_12_TB1010 | terminator, pos 5, lv0, 7th strongest |
| 249935 | pVC0_5_13_TDummy | terminator, pos 5, lv0, dummy |
| 249936 | pVC0_5a_04_C3xFLAG | C-terminal tag, lv0, 3xFLAG |
| 249937 | pVC0_5a_05_C6xHis | C-terminal tag, lv0, 6xHis |
| 249938 | pVC0_5a_06_CI11012 | C-terminal tag, lv0, I11012 |
| 249939 | pVC0_5a_07_CM0050 | C-terminal tag, lv0, M0050 |
| 249940 | pVC0_5a_08_CM0051 | C-terminal tag, lv0, M0051 |
| 249941 | pVC0_5a_09_CM0052 | C-terminal tag, lv0, M0052 |
| 249942 | pVC0_5a_10_CAzurite (Vn) | C-terminal tag, lv0, Azurite |
| 249943 | pVC0_5a_11_CmTurquoise (Vn) | C-terminal tag, lv0, mTurquoise |
| 249944 | pVC0_5a_13_CmVenus (Vn) | C-terminal tag, lv0, mVenus |
| 249945 | pVC0_5a_14_CmScarlet-I (Vn) | C-terminal tag, lv0, mScarlet-I |
| 249946 | pVC0_5a_15_CmCherry (Vn) | C-terminal tag, lv0, mCherry |
| 249947 | pVC0_5a_16_CmKate-2 (Vn) | C-terminal tag, lv0, mKate-2 |
| 249948 | pVC0_5a_17_CsfGFP(Vn)_new | C-terminal tag, lv0, sfGfp |
| 249949 | pVC0_5b_01_TB0010 | terminator, pos 5b, lv0, 2nd strongest |
| 249950 | pVC0_5b_02_TB0015 | terminator, pos 5b lv0, strongest |
| 249951 | pVC0_5b_03_TB1002 | terminator, pos 5b, lv0, 4th strongest |
| 249952 | pVC0_5b_04_TB1003 | terminator, pos 5b, lv0, 5th strongest |
| 249953 | pVC0_5b_05_TB1004 | terminator, pos 5b, lv0, 9th strongest |
| 249954 | pVC0_5b_06_TB1005 | terminator, pos 5b, lv0, 8th strongest |
| 249955 | pVC0_5b_07_TB1006 | terminator, pos 5b, lv0, 3rd strongest |
| 249957 | pVC0_5b_10_TB1009 | terminator, pos 5b, lv0, 10th strongest |
| 249958 | pVC0_5b_11_TB1010 | terminator, pos 5b, lv0, 7th strongest |
| 249959 | pVC0_5-6_01_3C1_operon | 3' operon connector, lv0, 3C1 |
| 249960 | pVC0_5-6_02_3C2_operon | 3' operon connector, lv0, 3C2 |
| 249961 | pVC0_5-6_03_3C3_operon | 3' operon connector, lv0, 3C3 |
| 249962 | pVC0_5-6_04_3C4_operon | 3' operon connector, lv0, 3C4 |
| 249963 | pVC0_5-6_05_3C5_operon | 3' operon connector, lv0, 3C5 |
| 249964 | pVC0_6_02_3C1SN | 3' connector, lv0, 3C1SN |
| 249965 | pVC0_6_05_3C1CSI | 3' connector, lv0, 3C1CSI |
| 249966 | pVC0_6_06_3C1RSI | 3' connector, lv0, 3C1RSI |
| 249967 | pVC0_6_07_3C2SN | 3' connector, lv0, 3C2SN |
| 249968 | pVC0_6_09_3C2SI | 3' connector, lv0, 3C2SI |
| 249970 | pVC0_6_12_3C3SI | 3' connector, lv0, 3C3SI |
| 249971 | pVC0_6_13_3C4SN | 3' connector, lv0, 3C4SN |
| 249972 | pVC0_6_15_3C4SI | 3' connector, lv0, 3C4SI |
| 249973 | pVC0_6_16_3C5CSN | 3' connector, lv0, 3C5CSN |
| 249974 | pVC0_6_17_3C5OSN | 3' connector, lv0, 3C5OSN |
| 249975 | pVC0_6_19_3C5SI | 3' connector, lv0, 3C5SI |
| 249976 | pVC0_6_20_3C0SN | 3' connector, lv0, 3C0SN |
| 249977 | pVC0_7_01_OColE1 | ORI, lv0, ColE1 |
| 249981 | pVC0_8_11_Atet_(sfgfp)(Vn) | ABR, lv0, Atet, sfGFP |
| 249983 | pVC0_8_13_Acam_(sfgfp)(Vn) | ABR, lv0, Acam, sfGFP |
| 249985 | pVC0_8_18_Akan(Vn)_(sfgfp)(Vn) | ABR, lv0, Akan, sfGFP |
| 249986 | pVC0_8_19_Akan(Vn)_(mScarlet)(Vn) | ABR, lv0, Akan, mScarlet-I |
| 249992 | pVC0_2_37_Dropout_BsaI_BpiI_mScarlet_BBa_J72163 GlpT | dropout part, lv0, pos 2, mScarlet-I |
| 249994 | pVC0_3_11_Dropout_BsaI_BpiI_mScarlet_BBa_J72163 GlpT | dropout part, lv0, pos 3, mScarlet-I |
| 249996 | pVC0_4_21_Dropout_BsaI_BpiI_mScarlet_BBa_J72163 GlpT | dropout part, lv0, pos 4, mScarlet-I |
| 249997 | pVC0_5_01_Dropout_BsaI_BpiI_sfGFP_BBa_J72163 GlpT | dropout part, lv0, pos 5, sfGFP |
| 249999 | pVC0_1-6_02_1-6_Connector | connector spanning pos 1-6, lv0 |
| 250000 | pVC_V*_01_LVL0* Entry sfGFP | Entry vector, standard, lv0*, sfGFP |
| 250002 | pVC_V*_03_LVL0* Res Entry sfGFP | Entry vector, ABR, lv0*, sfGFP |
| 250022 | pVC0*_A_03_Atet_(sfGFP) | ABR, lv0*, Atet, sfGFP |
| 250028 | pVC0*_A_12_Acarb J23106 (sfGFP) | ABR, lv0*, Acarb, sfGFP |
| 250029 | pVC0*_A_14_Acarb J23106 (mScarlet-I) | ABR, lv0*, Acarb, mScarlet-I |
| 250035 | pVC0*_EL_02_EL5C3-3C5O | endlinker, lv0*, 5C3-3C5O |
| 250038 | pVC0*_D_01_1R-5O sfGFP | dropout part, lv0*, 1C-5C, sfGFP |
| 250039 | pVC0*_D_02_1C-5C sfGFP | dropout part, lv0*, 1R-5O, sfGFP |
| 250040 | pVC0*_D_03_1R-5O mScarlet-I | dropout part, lv0*, 1C-5C, mScarlet-I |
| 250041 | pVC0*_D_04_1C-5C mScarlet-I | dropout part, lv0*, 1R-5O, mScarlet-I |
| 250045 | pVC2_D_06_tDNA_araA | tDNA plasmid dropout (araA) mScarlet copy 1 |
| 250046 | pVC2_D_07_tDNA_araFGH | tDNA plasmid dropout (araFGH) mScarlet |
| 250047 | pVC2_D_08_tDNA_fli | tDNA plasmid dropout (fli) mScarlet |
| 250050 | pVC2_D_11_tDNA_VNP1 | tDNA plasmid dropout (vps) mScarlet |
| 250051 | pVC2_D_12_tDNA_vps | tDNA plasmid dropout (vnp1) mScarlet |
| 250052 | pVC2_C_01_araA | NT-CRISPR plasmid (pST_116) with gRNA targetting phaBAC |
| 250054 | pVC2_C_03_fliR | NT-CRISPR plasmid (pST_116) with gRNA targetting vps |
| 250059 | pVC2_C_08_vps | NT-CRISPR plasmid (pST_116) with gRNA targetting mScarlet |