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pCODE tRNA Expression Plasmids Compensate for Rare Codons in Recombinant Protein Production.

Feller M, V Sepulchro AG, Murkoster M, Daley DO, Karyolaimos A, Jensen SI, Norholm MHH
ACS Synth Biol. 2026 Apr 21. doi: 10.1021/acssynbio.5c00794. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
247473pCODEPlasmid for constitutive expression of six tRNA encoding genes (argX, glyT, leuW, proL, argU, and ileX) in Escherichia coli, based on pSEVA121 backbone
247474pCODE-2Plasmid for constitutive expression of six tRNA encoding genes (argX, glyT, leuW, proL, argU, and ileX) in Escherichia coli, with p15A ori and Ampicillin resistance marker
247475pCODE-3Plasmid for inducible expression of six tRNA encoding genes (argX, glyT, leuW, proL, argU, and ileX) in Escherichia coli, based on pSEVA121 backbone

Antibodies from Article