Bipartite Genetically Encoded Biosensors to Sense Calcium Ion Dynamics at Membrane-Membrane Contact Sites.
Yamaguchi I, Barazzuol L, Dematteis G, Zhu W, Wen Y, Drobizhev M, Lim D, Campbell RE, Cali T, Nasu Y
Anal Chem. 2025 Sep 16;97(36):19848-19861. doi: 10.1021/acs.analchem.5c03831. Epub 2025 Sep 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 247995 | SPLICa:ER-MT (High Affinity) | Expresses the SPLICa reporter for ER-Mitochondria contacts and Calcium transients at the contact |
| 258609 | pcDNA-MBP-scpRFP1-P2A | Expresses self-complementing bipartite RFP in mammalian cells |
| 258610 | pcDNA-MBP-scpNG1-P2A | Expresses self-complementing bipartite mNeonGreen in mammalian cells |
| 258611 | pcDNA-MBP-scpGFP1-P2A | Expresses self-complementing bipartite GFP in mammalian cells |
| 258612 | pcDNA-sG-GECO1-P2A | Expresses bipartite GFP-based Ca2+ biosensor sG-GECO1 in mammalian cells |
| 258613 | pcDNA-sR-GECO1-P2A | Expresses bipartite RFP-based Ca2+ biosensor sR-GECO1 in mammalian cells |
| 258614 | pcDNA-sN-GECO1-P2A | Expresses bipartite mNeonGreen-based Ca2+ biosensor sN-GECO1 in mammalian cells |