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Experimental and simulated FRAP for the quantitative determination of protein diffusion in helical cells.

Sakib S, Fradin C
Methods Appl Fluoresc. 2026 Sep 9;14(4). doi: 10.1088/2050-6120/ae9b5b. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
248204pRK415-mNeonGreen (constitutive expression)Constitutive expression of mNeonGreen with a very low copy F-like plasmid under the control of a lac promoter. Plasmid contained within E. coli TOP10 (for cloning)
252211pRK415-mNeonGreen(inducible expression)Expresses mNeonGreen in a very low copy F-like plasmid under the inducible expression of a tac promoter within E. coli DH10Beta (for cloning)

Antibodies from Article