pET28g: A Golden Gate-compatible pET vector for protein expression in Escherichia coli, validated by production of functional human ACE2.
Luis IM, Parada M, Vicente JB, Abreu IA
PLoS One. 2025 Jul 7;20(7):e0327341. doi: 10.1371/journal.pone.0327341. eCollection 2025.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249630 | pET28g | Clone genes for protein expression in Escherichia coli using Golden Gate cloning and the pET28a backbone |
| 249631 | p028g13-MBP | Clone MBP polypeptide in the N-terminal of a protein to be expressed using pET28g backbone, using fusion sites 1 and 3 |
| 249632 | p028g13-Gb1 | Clone 8xHis-residues in tandem with Gb1 polypeptide in the N-terminal of a protein to be expressed using pET28g backbone, using fusion sites 1 and 3 |
| 249633 | p028g34-TEVs | Clone the cleavage site for TEV protease between the N-terminal tag and the CDS of the protein to be expressed using pET28g backbone, using fusion sites 3 and 4 |
| 249634 | p028g13-6His | Clone 6xHis-residues in the N-terminal of a protein to be expressed using pET28g backbone, using fusion sites 1 and 3 |
| 249635 | p028g13-8His | Clone 8xHis-residues in the N-terminal of a protein to be expressed using pET28g backbone, using fusion sites 1 and 3 |
| 249636 | p028g34-3Cs | Clone the cleavage site for 3C protease between the N-terminal tag and the CDS of the protein to be expressed using pET28g backbone, using fusion sites 3 and 4 |