Fast, Bright, and Reversible Fluorescent Labeling of Rhodamine-Binding Proteins.
Kompa J, Dornfeld LJ, Porzberg N, Jang S, Zedlitz S, Lilje SH, Catapano C, Jocher D, Merk L, Hoege C, Mao R, Wilhelm J, Dietz MS, Tarnawski M, Hiblot J, Hyman AA, Heilemann M, Johnsson K
J Am Chem Soc. 2025 Dec 30. doi: 10.1021/jacs.5c18083.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249665 | pET-51b(+)_His10x-TEV-Rho-tag | Expression of rhodamine-binding protein tag in bacterial cells |
| 249666 | pET-51b(+)_His10x-TEV-SiR-tag | Expression of silicon rhodamine-binding protein tag in bacterial cells |
| 249667 | pcDNA5/FRT/TO_H2B-Rho-tag-T2A-mEGFP | Co-expression of rhodamine-binding protein tag (nuclear) and mEGFP (not localized) in mammalian cells |
| 249668 | pcDNA5/FRT/TO_H2B-SiR-tag-T2A-mEGFP | Co-expression of silicon rhodamine-binding protein tag (nuclear) and mEGFP (not localized) in mammalian cells |
| 249669 | pcDNA5/FRT/TO_CD86-Rho-tag | Expression of monomeric rhodamine-binding protein tag on the cell surface in mammalian cells |
| 249671 | pTol2-HuC_Rho-tag-Pro30-mEGFP-NLS3x | Expression of rhodamine-binding protein tag fused to mEGFP (nuclear) in zebrafish |
| 249672 | pTol2-HuC_SiR-tag-Pro30-mEGFP-NLS3x | Expression of silicon rhodamine-binding protein tag fused to mEGFP (nuclear) in zebrafish |