RNA localization to nuclear speckles follows splicing logic.
Wen L, Arias MA, Fan X, Paul S, Liao SE, Sobczyk M, Regev O, Fei J
bioRxiv [Preprint]. 2025 May 28:2025.05.28.656493. doi: 10.1101/2025.05.28.656493.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249737 | M_3SR5HN | Minimal synthetic construct for assessing speckle localization containing a 3' splice site, SR motif-rich region, 5' splice site, and an HNRNP motif-rich region |
| 249738 | M_3SRHN | Minimal synthetic construct for assessing speckle localization containing a 3' splice site, SR motif-rich region, and an HNRNP motif-rich region |
| 249739 | M_SR5HN | Minimal synthetic construct for assessing speckle localization containing an SR motif-rich region, 5' splice site, and an HNRNP motif-rich region |
| 249740 | M_SRHN | Minimal synthetic construct for assessing speckle localization containing an SR motif-rich region and an HNRNP motif-rich region |
| 249741 | M_3HN5SR | Minimal synthetic construct for assessing speckle localization containing a 3' splice site, an HNRNP motif-rich region, 5' splice site, and an SR motif-rich region |
| 249742 | M_HN5SR | Minimal synthetic construct for assessing speckle localization containing an HNRNP motif-rich region, 5' splice site, and an SR motif-rich region |
| 249743 | M_3HNSR | Minimal synthetic construct for assessing speckle localization containing a 3' splice site, an HNRNP motif-rich region, and an SR motif-rich region. |
| 249744 | M_HNSR | Minimal synthetic construct for assessing speckle localization containing an HNRNP motif-rich region and an SR motif-rich region |
| 249745 | M_3SR5 | Minimal synthetic construct for assessing speckle localization containing a 3' splice site, SR motif-rich region, and a 5' splice site |
| 249746 | M_SR5 | Minimal synthetic construct for assessing speckle localization containing an SR motif-rich region and a 5' splice site |
| 249747 | M_3SR | Minimal synthetic construct for assessing speckle localization containing a 3' splice site and an SR motif-rich region |
| 249748 | M_SR | Minimal synthetic construct for assessing speckle localization containing an SR motif-rich region |
| 249749 | M_3HN5 | Minimal synthetic construct for assessing speckle localization containing a 3' splice site, an HNRNP motif-rich region, and a 5' splice site |
| 249750 | M_HN5 | Minimal synthetic construct for assessing speckle localization containing an HNRNP motif-rich region and a 5' splice site |
| 249751 | M_3HN | Minimal synthetic construct for assessing speckle localization containing a 3' splice site and an HNRNP motif-rich region |
| 249752 | M_HN | Minimal synthetic construct for assessing speckle localization containing an HNRNP motif-rich region |
| 249753 | M_5SR | Minimal synthetic construct for assessing speckle localization containing a 5' splice site and an SR motif-rich region |
| 249754 | M_SR3 | Minimal synthetic construct for assessing speckle localization containing an SR motif-rich region and a 3' splice site |
| 249755 | M_5HN | Minimal synthetic construct for assessing speckle localization containing a 5' splice site and an HNRNP motif-rich region |
| 249756 | M_HN3 | Minimal synthetic construct for assessing speckle localization containing an HNRNP motif-rich region and a 3' splice site |
| 249757 | M_A1 | Minimal synthetic construct for assessing speckle localization, part of A trajectory series (starting with M_3HN5 and ending in M_3SR5) |
| 249758 | M_A2 | Minimal synthetic construct for assessing speckle localization, part of A trajectory series (starting with M_3HN5 and ending in M_3SR5) |
| 249759 | M_A3 | Minimal synthetic construct for assessing speckle localization, part of A trajectory series (starting with M_3HN5 and ending in M_3SR5) |
| 249760 | M_A4 | Minimal synthetic construct for assessing speckle localization, part of A trajectory series (starting with M_3HN5 and ending in M_3SR5) |
| 249761 | M_A5 | Minimal synthetic construct for assessing speckle localization, part of A trajectory series (starting with M_3HN5 and ending in M_3SR5) |
| 249762 | M_A6 | Minimal synthetic construct for assessing speckle localization, part of A trajectory series (starting with M_3HN5 and ending in M_3SR5) |
| 249763 | M_B1 | Minimal synthetic construct for assessing speckle localization, part of B trajectory series (starting with M_3HN5 and ending in M_3SR5) |
| 249764 | M_B2 | Minimal synthetic construct for assessing speckle localization, part of B trajectory series (starting with M_3HN5 and ending in M_3SR5) |
| 249765 | M_B3 | Minimal synthetic construct for assessing speckle localization, part of B trajectory series (starting with M_3HN5 and ending in M_3SR5) |
| 249766 | M_B4 | Minimal synthetic construct for assessing speckle localization, part of B trajectory series (starting with M_3HN5 and ending in M_3SR5) |
| 249767 | M_B5 | Minimal synthetic construct for assessing speckle localization, part of B trajectory series (starting with M_3HN5 and ending in M_3SR5) |
| 249768 | M_B6 | Minimal synthetic construct for assessing speckle localization, part of B trajectory series (starting with M_3HN5 and ending in M_3SR5) |
| 249769 | M_3SRweak5 | Minimal synthetic construct for assessing speckle localization, derived from M_3SR5, with 5'ss weakened |
| 249770 | M_noAG_3SR5 | Minimal synthetic construct for assessing speckle localization, derived from M_3SR5, with canonical AG dinucleotide removed |
| 249771 | M_weakPY_3SR5 | Minimal synthetic construct for assessing speckle localization, derived from M_3SR5, with polypyrimidine weakened |
| 249772 | M_noBP_3SR5 | Minimal synthetic construct for assessing speckle localization, derived from M_3SR5, with branchpoint removed |
| 249773 | M_SMN1 | Minimal construct for assessing speckle localization containing SMN1 exon 7 |
| 249774 | M_SMN2 | Minimal construct for assessing speckle localization containing SMN2 exon 7 |
| 249775 | M_COLQ_WT | Minimal construct for assessing speckle localization containing COLQ exon 16 |
| 249776 | M_COLQ_MUT | Minimal construct for assessing speckle localization containing COLQ exon 16 (c.1244A>G) |
| 249777 | M_5HN_upstream_cryptic_AG_removed | Minimal synthetic construct for assessing speckle localization, derived from M_5HN |
| 249778 | M_5HN_upstream_cryptic_PY_removed | Minimal synthetic construct for assessing speckle localization, derived from M_5HN |
| 249779 | M_3SR5SR | Minimal synthetic construct for assessing speckle localization containing a 3' splice site, SR motif-rich region, 5' splice site, and a second SR motif-rich region |
| 249780 | M_3HN5HN | Minimal synthetic construct for assessing speckle localization containing a 3' splice site, HNRNP motif-rich region, 5' splice site, and a second HNRNP motif-rich region |
| 249781 | F_3SR5HN | M_3SR5HN cloned into an intron flanked by two exons |
| 249782 | F_3SRHN | M_3SRHN cloned into an intron flanked by two exons |
| 249783 | F_SR5HN | M_SR5HN cloned into an intron flanked by two exons |
| 249784 | F_SRHN | M_SRHN cloned into an intron flanked by two exons |
| 249785 | F_3HN5SR | M_3HN5SR cloned into an intron flanked by two exons |
| 249786 | F_HN5SR | M_HN5SR cloned into an intron flanked by two exons |
| 249788 | F_HNSR | M_HNSR cloned into an intron flanked by two exons |
| 249789 | F_3SR5 | M_3SR5 cloned into an intron flanked by two exons |
| 249790 | F_SR5 | M_SR5 cloned into an intron flanked by two exons |
| 249791 | F_3SR | M_3SR cloned into an intron flanked by two exons |
| 249792 | F_SR | M_SR cloned into an intron flanked by two exons |
| 249793 | F_3HN5 | M_3HN5 cloned into an intron flanked by two exons |
| 249794 | F_HN5 | M_HN5 cloned into an intron flanked by two exons |
| 249795 | F_3HN | M_3HN cloned into an intron flanked by two exons |
| 249796 | F_HN | M_HN cloned into an intron flanked by two exons |
| 249797 | F_A1 | M_A1 cloned into an intron flanked by two exons |
| 249798 | F_A2 | M_A2 cloned into an intron flanked by two exons |
| 249799 | F_A3 | M_A3 cloned into an intron flanked by two exons |
| 249800 | F_A4 | M_A4 cloned into an intron flanked by two exons |
| 249801 | F_A5 | M_A5 cloned into an intron flanked by two exons |
| 249802 | F_A6 | M_A6 cloned into an intron flanked by two exons |
| 249803 | F_B1 | M_B1 cloned into an intron flanked by two exons |
| 249804 | F_B2 | M_B2 cloned into an intron flanked by two exons |
| 249805 | F_B3 | M_B3 cloned into an intron flanked by two exons |
| 249806 | F_B4 | M_B4 cloned into an intron flanked by two exons |
| 249807 | F_B5 | M_B5 cloned into an intron flanked by two exons |
| 249808 | F_B6 | M_B6 cloned into an intron flanked by two exons |
| 249809 | U1_snRNA_WT_SS | Wildtype human U1 snRNA |
| 249810 | U1_snRNA_Weak_SS | Mutated human U1 snRNA complementary to a weak 5' splice site sequence CAGgtacga |