Lysosomal down-regulation of the mu opioid receptor is opposed by the Retromer complex.

Dagunts A, Adoff H, Novy B, Ameur LB, De Maria M, Saunders A, Lobingier BT
Sci Adv. 2026 Mar 20;12(12):eadx8715. doi: 10.1126/sciadv.adx8715. Epub 2026 Mar 20. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
250759	puDNA5_SSF_MOR_WT_APEX2	Mammalian cell expression of the wildtype mu opioid receptor
250760	puDNA5_SSF_MOR_2Ala_APEX2	Mammalian cell expression of the mu opioid receptor with L387 and L390 mutated to alanines
250762	puDNA5_SSF_DOR_MRS_WT_APEX2	Mammalian cell expression of the delta opioid receptor with the last 17 amino acids of the mu opioid receptor
250763	puDNA5_SSF_DOR_MRS_2Ala_APEX2	Mammalian cell expression of the delta opioid receptor with the last 17 amino acids of the mu opioid receptor, in which the leucines in the LENL sequence have been mutated to alanines
250764	pLenti_H1_Scramble_shRNA_UBC_SSF_SNAP_MOR_WT_P2A_GFP	Mammalian cell expression of a scrambled shRNA and the mu opioid receptor
250765	pLenti_H1_VPS35_shRNA_UBC_SSF_SNAP_MOR_WT_P2A_GFP	Mammalian cell expression of an shRNA against VPS35 and the mu opioid receptor
250766	pcDNA5_TO_FRT_myc_GLUT4_WT_APEX2	Mammalian cell expression of GLUT4
250767	pcDNA5_TO_FRT_myc_GLUT4_2Ala_APEX2	Mammalian cell expression of GLUT4 in which L500 and L503 are mutated to alanines
250768	puDNA5_SSF_DOR_GLUT4_LEYL_APEX2	Mammalian cell expression of the delta opioid receptor with the last 17 amino acids of GLUT4
250769	puDNA5_SSF_DOR_GLUT4_AEYL_APEX2	Mammalian cell expression of the delta opioid receptor with the last 17 amino acids of GLUT4, in which the first leucine in the "LEYL" sequence is mutated to alanine
250770	puDNA5_SSF_DOR_GLUT4_LEAL_APEX2	Mammalian cell expression of the delta opioid receptor with the last 17 amino acids of GLUT4, in which the first leucine in the "LEYL" sequence is mutated to alanine
250771	puDNA5_SSF_DOR_GLUT4_LEYA_APEX2	Mammalian cell expression of the delta opioid receptor with the last 17 amino acids of GLUT4, in which the second leucine in the "LEYL" sequence is mutated to alanine
250772	puDNA5_SSF_DOR_GLUT4_AEYA_APEX2	Mammalian cell expression of the delta opioid receptor with the last 17 amino acids of GLUT4, in which both leucines in the "LEYL" sequence are mutated to alanine
250773	pLenti_SSF_MOR_WT_APEX2	Mammalian cell expression of the mu opioid receptor
250774	pLenti_SSF_MOR_2Ala_APEX2	Mammalian cell expression of the mu opioid receptor with L387 and L390 mutated to alanines