Lior Nissim Lab: Modular Scalable Synthetic Gene Circuits for Complex Functions Within Minimal Computational Layers in Human Cells
Nissim Lab
unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 251264 | pKR3:SSX1p-mK2-Ex1-(5'D)-ID-AFP BD-S(50bp, 30%GC)-(linker1)-pA | Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits |
| 251265 | pKR4:H2A1p-(linker2)-AFP BD-AS(50bp)2mm(18-19)-(linker3)-(AFP 3'SE)-mK2-Ex2-(linker4)-pA | Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251266 | pKR5:H2A1p-(linker2)-AFP BD-AS(1-50bp)2mm(18-19)-(linker3)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA | Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251267 | pKR7:H2A1p-(linker2)-AFP BD-AS(50bp)2mm(18-19)-(linker3)-(Canonical 3'SE V2 mutated PPT)-mK2-Ex2-(linker4)-pA | Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251268 | pKR9:H2A1p-(linker2)-BD AFP-AS(21-50bp complement)-(linker3)-(AFP 3'SE)-mK2-Ex2-(linker4)-pA | Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251269 | pKR10:SSX1p-mK-Ex1-(5'D)-AFP BD-S (21-50bp)-(linker1)-pA | Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits |
| 251270 | pKR12:H2A1p-(linker2)-AFP BD-AS (1-30bp complement)-(linker3)-(AFP 3'SE)-mK2-Ex2-(linker4)-pA | Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251271 | pKR13:SSX1p-mK2-Ex1-(5'D)-ID-AFP BD-S (1-30bp)-(linker1)-pA | Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits |
| 251272 | pKR14:SSX1p-mK2-Ex1-(5'D)-ID-AFP BD-S (50bp)-(linker5[20bp])-AFP BD-S (50bp, 30%GC)-(linker1)-pA | Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits |
| 251273 | pKR15:SSX1p-mK2-Ex1-(5'D)-ID-BD1-S(50bp, 50%GC)-(linker1)-pA | Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits |
| 251274 | pKR16:H2A1p-(linker2)-BD1-AS(50bp complement)-(linker3)-(AFP 3'SE)-mK2-Ex2-(linker4)-pA | Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251275 | pKR17:SSX1p-mK2-Ex1-(5'D)-ID-BD2-S(50bp 30%GC)-(linker1)-pA | Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits |
| 251276 | pKR18:H2A1p-(linker2)-BD2-AS (50bp complement)-(linker3)-(AFP 3'SE)-mK2-Ex2-(linker4)-pA | Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251277 | pKR19:SSX1p-mK2-Ex1-(5'D)-ID-BD3-S-(30bp 50%GC)-(linker1)-pA | Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits |
| 251278 | pKR20:H2A1p-(linker2)-BD3-AS (30bp complement)-(linker3)-(AFP 3'SE)-mK2-Ex2-(linker4)-pA | Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251279 | pKR21:SSX1p-mK2-Ex1-(5'D)-ID-BD4-S-(30bp 30%GC)-(linker1)-pA | Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits |
| 251280 | pKR22:H2A1p-(linker2)-BD4-AS (30bp complement)-(linker3)-(AFP 3'SE)-mK2-Ex2-(linker4)-pA | Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251281 | pKR38:H2A1p-(linker2)-AFP BD-AS(50bp)mm(18,19)-(linker3)-(mutated BP Canonical 3'SE V3)-mK2-Ex2-(linker4)-pA | Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251282 | pKR69:H2A1p-(linker2 [10bp])-AFP BD-AS(50bp)2mm(18-19)-(linker3)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA | Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251283 | pKR71:H2A1p-(linker2)-AFP BD-AS(50bp)2mm(18-19)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA | Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251284 | pKR72:H2A1p-AFP BD-AS(50bp)2mm(18-19)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA | Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251285 | pKR73:SSX1p-mK2-Ex1-(5'D)-AFP BD-S(50bp, 30%GC)-(linker1)-pA | Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits |
| 251286 | pKR74:SSX1p-mK2-Ex1-(5'D)-ID-AFP BD-S(50bp, 30%GC)-pA | Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits |
| 251287 | pKR75:SSX1p-mK2-Ex1-(5'D)-AFP BD-S(50bp, 30%GC)-pA | Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits |
| 251288 | pKR78:SSX1p-mK2-Ex1-(5'D)-ID-BD6-S (50bp)-pA | Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits |
| 251289 | pKR80:H2A1p-BD6-AS (50bp)2mm(18-19)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA | Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251290 | pKR81:SSX1p-mK2-Ex1-(5'D)-ID-BD5-S(50bp, 50%GC)-pA | Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits |
| 251291 | pKR83:H2A1p-BD5-AS(50bp, 50%GC)2mm(18-19)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA | Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251292 | pKR84:SSX1p-mK2-Ex1-(5'D)-ID-BD7-S (30bp, 36%GC)-pA | Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits |
| 251293 | pKR85:H2A1p-BD7-AS (30bp)2mm(12-13)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA | Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251294 | pKR86:SSX1p-mK2-Ex1-(5'D)-ID-BD8-S (30bp, 36%GC)-pA | Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits |
| 251295 | pKR87:H2A1p-BD8-AS (30bp)2mm(12-13)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA | Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251296 | pKR88:SSX1p-mK2-Ex1-(5'D)-ID-BD9-S (30bp, 36%GC)-pA | Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits |
| 251297 | pKR89:H2A1p-BD9-AS (30bp)2mm(12-13)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA | Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251298 | pKR90:SSX1p-mK2-Ex1-(5'D)-ID-BD10-S (30bp, 30%GC)-pA | Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits |
| 251299 | pKR91:H2A1p-BD10-AS (30bp)2mm(12-13)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA | Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251300 | pRZ111:G8p-EYFP-pA | Expression of EYFP under G8p promoter for use in synthetic gene circuits |
| 251301 | pRZ134:CMVp-BD9-AS-(3'SE)-GAD-EX2-pA | Expression of GAD-Ex2 under CMVp promoter for use in synthetic gene circuits |
| 251302 | pRZ135:CMVp-GAD-EX1-(5'D)-BD9-S-pA | Expression of GAD-Ex1 under CMVp promoter for use in synthetic gene circuits |
| 251303 | pRZ138:CMVp-GAD-Ex1-(5'D)-BD6-S-pA | Expression of GAD-Ex1 under CMVp promoter for use in synthetic gene circuits |
| 251304 | pRZ139:CMVp-EC-Ex1-(5'D)-BD5-S-pA | Expression of ECFP-Ex1 under CMVp promoter for use in synthetic gene circuits |
| 251305 | pRZ145:CMVp-BD5-AS-(3'SE)-EC-Ex2-pA | Expression of ECFP-Ex2 under CMVp promoter for use in synthetic gene circuits |
| 251306 | pRZ146:CMVp-BD6-AS-(3'SE)-GAD-Ex2-pA | Expression of GAD-Ex2 under CMVp promoter for use in synthetic gene circuits |
| 251307 | pRZ150:CMVp-mK2-Ex1-miRS1-mK2-Ex2-PEST-miRS2-BSs-miRS3-BSs-pA | Expression of mKate2 under CMVp promoter for use in synthetic gene circuits |
| 251308 | pRZ151:CMVp-BD9-AS-(3'SE)-EC-Ex2-pA | Expression of ECFP-Ex2 under CMVp promoter for use in synthetic gene circuits |
| 251309 | pRZ153:CMVp-EC-Ex1-(5'D)-BD9-S-pA | Expression of ECFP-Ex1 under CMVp promoter for use in synthetic gene circuits |
| 251310 | pRZ171:CMVe-G3BSs-TATA-mK2-Ex1-miRS2-mK2-Ex2-miRS1-BSs-miRS3-BSs-pA | Expression of mKate2 under CMVe promoter for use in synthetic gene circuits |
| 251311 | pRZ180:[pA-BD9-S-BD5-S-(5'D)-EC-Ex1]-CMVp-[(G3p)RC-mK2-Ex1-miRS2-mK2-Ex2-miRS1-BSs-miRS3-BSs-pA] | Expression of mKate2 Exon 1 and 2 and ECFP-Ex1 for use in synthetic gene circuits |
| 251312 | pRZ212:CMVp-GAD-pA | Expression of GAD under CMVp promoter for use in synthetic gene circuits |
| 251313 | pRZ230:CMVe-G3BSs-TATA-mKate2-pA | Expression of mKate2 under CMVe promoter for use in synthetic gene circuits |
| 251314 | pRZ231:Inactive Control-G3BSs-TATA-mKate2-pA | Inactive Control for mKate2 for use in synthetic gene circuits |
| 251315 | pRZ232:CMVe-Spacer-TATA-mKate2-pA | Expression of mKate2 under CMVe promoter for use in synthetic gene circuits |
| 251316 | pRZ333:CMVp-Sirius-miRS1-BSs-miRS2-BSs | Expression of Sirius under CMVp promoter for use in synthetic gene circuits |
| 251317 | pRZ234:H2A1p-BD6-AS-3'SE-mK-EX2-3*miRS1-BS-pA | Expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251318 | pRZ235:CMVp-EC-EX1-miRS1-EC-EX2-pA | Expression of ECFP under CMVp promoter for use in synthetic gene circuits |
| 251319 | pRZ241:SSX1p-GAD-EX1-5'D-ID-BD6-S(50bp) | Expression of GAD-EX1 under SSX1p promoter for use in synthetic gene circuits |
| 251320 | pRZ242:H2A1p-BD6-AS(50bp)-Canonical 3'SE V1-GAD-EX2-linker4-pA | Expression of GAD-EX2 under H2A1p promoter for use in synthetic gene circuits |
| 251321 | pRZ274:SSX1p-mK2-Ex1-ID-AFP BD-S-(linker1)-pA | Expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits |
| 251322 | pRZ275:H2A1-linker2-AFP BD-AS-linker3-mK2-Ex2-linker4-pA | Expression of mK2-Ex2 under H2A1 promoter for use in synthetic gene circuits |
| 251323 | pRZ314:CMVp-mK2-Ex1-(5'D)-ID-BD6-S(50bp)-pA | Expression of mK2-Ex1 under CMVp promoter for use in synthetic gene circuits |
| 251324 | pRZ315:CMVp-BD6-AS (50bp)2mm(18-19)-(Canonical V13'SE)-mK2-Ex2-(linker4)-pA | Expression of mK2-Ex2 under CMVp promoter for use in synthetic gene circuits |
| 251325 | pRZ330:CMVp-mK-Ex1-miRS3-mK-Ex2-PEST-miRS1-BSs-miRS2-BSs-pA | Expression of mKate2 under CMVp promoter for use in synthetic gene circuits |
| 251326 | pRZ334:CMVp-BD6-AS-(3'SE)-EC-Ex2-miRS2-BSs-pA | Expression of ECFP-Ex2 under CMVp promoter for use in synthetic gene circuits |
| 251327 | pRZ335:CMVp-BD5-AS-(3'SE)-mK2-Ex2-miRS1-BSs-pA | Expression of mK2-Ex2 under CMVp promoter for use in synthetic gene circuits |
| 251328 | pRZ336:CMVp-EC-Ex1-(5'D)-miRS1-(3'SE)-BD6-S-pA | Expression of ECFP-Ex1 under CMVp promoter for use in synthetic gene circuits |
| 251329 | pRZ337:CMVp-mK2-Ex1-(5'D)-miRS2-(3'SE)-BD5-S-pA | Expression of mK2-Ex1 under CMVp promoter for use in synthetic gene circuits |
| 251330 | pRZ365:CMVe-Spacer(80bp)-TATA-mK2-Ex1-miRS2-mK2-Ex2-miRS1-BSs-pA | Expression of mKate2 under CMVe promoter for use in synthetic gene circuits |
| 251331 | pRZ366:CMVp-mK2-Ex1-miRS1-mK2-Ex2-PEST-miRS2-BSs-pA | Expression of mKate2 under CMVp promoter for use in synthetic gene circuits |
| 251332 | pRZ385:CMVp-EC-Ex1-(5'D)-ID-BD6-S(50bp)-pA | Expression of ECFP-Ex1 under CMVp promoter for use in synthetic gene circuits |
| 251333 | pRZ386:CMVp-BD6-AS (50bp)2mm(18-19)-(Canonical 3'SE V1)-EC-Ex2-pA | Expression of ECFP-Ex2 under CMVp promoter for use in synthetic gene circuits |
| 251334 | pRZ427:Inactive Control-mKate2 | Inactive Control of mKate2 for use in synthetic gene circuits |
| 251335 | pRZ432:SSX1p-mK2-Ex1-(5'D)-ID-(linker1)-pA | Expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits |
| 251336 | pRZ433:H2A1p-(linker2)-(linker3)-(AFP 3'SE)-mK2-Ex2-(linker4)-pA | Expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits |
| 251337 | pRZ500:G5p-mKate2-pA | Expression of mKate2 under G5p promoter for use in synthetic gene circuits |
| 251338 | pRZ501:G5p-IL15-pA | Expression of IL-15 under G5p promoter for use in synthetic gene circuits |