Hydrophobic tuning with non-canonical amino acids in a copper metalloenzyme.
Fischer S, Natter Perdiguero A, Lau K, Deliz Liang A
Nat Chem. 2026 Apr 13. doi: 10.1038/s41557-026-02116-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 251554 | pGS1T-PylRSC6a-PylTm15CUA | Production of engineered aaRS/tRNA pair for C6a incorporation, also suitable for C5a and C7a incorporation |
| 251555 | pGS1T-PylRSC6a-MmtRNAPylCUA | Production of engineered aaRS/tRNA pair for C6a incorporation with lower yield but low background in the absence of C6a |
| 251556 | pET28(+)-SLAC-6xHis | Production of the small laccase from Streptomyces coelicolor with C-terminal His6 and missing the N-terminal signaling peptide |