A split ALFA tag-nanobody system for protein localization and proximity proteomics in mycobacteria.
Fay A, Kurland AP, Li Z, Monetti M, Johnson JR, Glickman MS
mBio. 2025 Aug 13;16(8):e0097125. doi: 10.1128/mbio.00971-25. Epub 2025 Jun 27.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 252903 | pAJF1120 | Tet-inducible GFP-NBALFA fusion plasmid. Contains oriM and kanamycin-resistance cassette. |
| 252904 | pAJF1124 | 3' rpoC(MSMEG_1368)-ALFA for rpoC-ALFA in M. smegmatis via single crossover. Encodes hygromycin-resistance cassette. |
| 252906 | pAJF1131 | T7-pelB-NBALFA-LE-6his amp (for purification of his-tagged NBALFA from E. coli) |
| 252907 | pAJF1137 | Tet-inducible TurboID-NBALFA fusion plasmid. Contains oriM (mycobacterial origin of replication) and kanamycin-resistance cassette. |
| 252908 | pAJF1139 | For constitutive expression of GFP-NBALFA. Contains oriM (mycobacterial origin of replication) and kanamycin-resistance cassette. |
| 252909 | pAJF1141 | For constitutive expression of GFP-NBALFA. Contains oriM (mycobacterial origin of replication) and kanamycin-resistance cassette. |