Exapted CRISPR-Cas12f homologues drive RNA-guided transcription.
Hoffmann FT, Wiegand T, Palmieri AI, Glass-Klaiber J, Xiao R, Tang S, Le HC, Meers C, Lampe GD, Chang L, Sternberg SH
Nature. 2026 Mar 4. doi: 10.1038/s41586-026-10166-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 254269 | pSL7458 (Fta RNA polymerase) | Encodes E. coli codon-optimized rpoA, rpoB, rpoC, and rpoZ from Flagellimonas taeanensis driven by an inducible T7 promoter |
| 254270 | pSL8106 (dCas12f–σE (sigmaE) system, yidX guide) | Encodes E. coli codon-optimized dCas12f and σE (sigmaE) from Flagellimonas taeanensis driven by constitutive promoters, with guide targeting E. coli yidX gene |
| 254271 | pSL8055 (dCas12f–σE (sigmaE) system, native Fta guide) | Encodes E. coli codon-optimized dCas12f and σE (sigmaE) from Flagellimonas taeanensis driven by constitutive promoters with guide targeting native Fta gene |
| 254272 | pSL8215 (dCas12f–σE (sigmaE) system, entry vector) | Encodes E. coli codon-optimized dCas12f and σE (sigmaE) from Flagellimonas taeanensis driven by constitutive promoters, with BsaI-containing restriction sites for guide RNA cloning |