Development of pXeDNA3.1-LacZ, a Dual-System Plasmid for Membrane Protein Expression in Xenopus and Mammalian Cells.

Guan L, Reynoldson TE, Pieroni EM, Dillon JC, Hardege I, Yanez-Guerra LA
MicroPubl Biol. 2025 Dec 1;2025:10.17912/micropub.biology.001866. doi: 10.17912/micropub.biology.001866. eCollection 2025. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
242746	pXeDNA3.1-LacZ	pXeDNA3-LacZ TA-cloning vector for dual expression: CMV enhancer/promoter in mammalian cells and Xenopus β-globin 5'/3'UTRs in oocytes; BGHpA, AmpR, NeoR, lacZα for blue-white screening.
244454	pXeDNA3.1-HsTRPV1	pXeDNA3.1-HsTRPV1 encodes human TRPV1 in CMV/Xenopus β‑globin UTR scaffold for robust expression in mammalian cells and Xenopus oocytes; BGH polyA, AmpR, NeoR, lacZα for blue‑white screening.