MiR-513a promotes human erythroid differentiation by modulating c-Jun.
Kim M, Taylor B, Bolten S, Eberly CL, Abdurahman M, Creed TM, Yang A, Hatchet TL, Dyson T, Gu S, Civin CI, Kingsbury TJ
FEBS Open Bio. 2026 Mar 8. doi: 10.1002/2211-5463.70226.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 254384 | pWCC52 | Empty lentivector to overexpress microRNA |
| 254385 | pTJK6 (miR-513a WT OE in pWCC52) | Express human miR-513a |
| 254386 | pTJK281 (miR-513a-5p mut OE in pWCC52) | Express human miR-513a-5p mutant |
| 254387 | pTJK282 (miR-513a-3p mut OE in pWCC52) | Express human miR-513a-3p mutant |
| 254388 | pWCC43 | Empty lentivector to overexpress gene of interest |
| 254389 | c-JUN OE in pWCC43 | Express human c-JUN |
| 254390 | c-JUN KO in LentiCRISPRv2GFP | Express sgRNA targeting human c-JUN |
| 254391 | c-JUN partial 3'UTR (WT) in pmiRGLO Dual-Luciferase | Express luciferase reporter containing partial human c-JUN 3'UTR (wild type) |
| 254392 | c-JUN partial 3'UTR (mut) in pmiRGLO Dual-Luciferase | Express luciferase reporter containing partial human c-JUN 3'UTR mutant (with the predicted miR-513a-5p binding site deleted) |
| 254393 | pTJK689 (EPOR KO in LentiCRISPRv2GFP) | Express sgRNA targeting human EPOR |