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SARS-CoV-2 resistance pathways to EDP-235.

Wang K, Gibbons JS, Bisht N, Reyes AC, Cressey TN, Crepeau ML, Balakrishnan A, Kelly NM, Bai T, Lovett DH, Torres Yanagisawa AE, Caccin FNM, Rhodin MHJ, Iketani S
Antiviral Res. 2026 Sep;253:106493. doi: 10.1016/j.antiviral.2026.106493. Epub 2026 Jul 27. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
254472pLEX307-puro-SARS-CoV-2-nsp4-nsp5-P132S-nsp6Constitutive expression of SARS-CoV-2 nsp5 with P132S mutation fused to nsp4 and an N-terminal fragment of nsp6.
254473pLEX307-puro-SARS-CoV-2-nsp4-nsp5-P168S-nsp6Constitutive expression of SARS-CoV-2 nsp5 with P168S mutation fused to nsp4 and an N-terminal fragment of nsp6.
254474pLEX307-puro-SARS-CoV-2-nsp4-nsp5-L50F-E166A-nsp6Constitutive expression of SARS-CoV-2 nsp5 with L50F and E166A mutations fused to nsp4 and an N-terminal fragment of nsp6.
254475pLEX307-puro-SARS-CoV-2-nsp4-nsp5-L50F-E166A-P168S-nsp6Constitutive expression of SARS-CoV-2 nsp5 with L50F, E166A, and P168S mutations fused to nsp4 and an N-terminal fragment of nsp6.

Antibodies from Article