Alexander Brown Lab: Laboratory for Genomics Research - Human CRISPR Pooled Libraries
Brown
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 254656 | hCRISPRi v2 | Human genome-scale CRISPRi library for inhibition of gene transcription with a pLGR1002 backbone. |
| 254657 | hCRISPRa v2 | Human genome-scale CRISPRa library for activation of gene transcription with a pLGR1002 backbone. |
| 254658 | hCRISPRi v3 | Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette with a pLGR_dual_guide-Puro-mycNLS-BFP backbone. |
| 254659 | ”Brunello” hCRISPR KO – BFP | Human CRISPR knockout library with pLGR_dual_guide-Puro-mycNLS-BFP backbone, designed using optimized metrics which combined improved on-target activity predictions (Rule Set 2), with an off-target sc |
| 254660 | ”Brunello” hCRISPR KO – eGFP | Human CRISPR knockout library with pLGR_dual_guide-Puro-mycNLS-eGFP backbone, designed using optimized metrics which combined improved on-target activity predictions (Rule Set 2), with an off-target s |
| 254661 | ”Brunello” hCRISPR KO - mCherry | Human CRISPR knockout library with pLGR_dual_guide-Puro-mycNLS-mCherry backbone, designed using optimized metrics which combined improved on-target activity predictions (Rule Set 2), with an off-targe |