Jonathan Calles Lab: Protein Synthesis Using Recombinant Elements (PURE) cell-free transcription/translation system in E. coli

b.next
unpublished

Plasmids from Article

ID	Plasmid	Purpose
259937	pET28a-AK-Gg	Expression vector for producing chicken adenylate kinase 1 (AK), a component of the PURE cell-free transcription/translation system, in E. coli.
259938	pET28a-AlaRS	Expression vector for producing E. coli alanyl-tRNA synthetase (AlaRS), a component of the PURE cell-free transcription/translation system, in E. coli.
259940	pET28a-AsnRS	Expression vector for producing E. coli asparaginyl-tRNA synthetase (AsnRS), a component of the PURE cell-free transcription/translation system, in E. coli.
259941	pET28a-AspRS	Expression vector for producing E. coli aspartyl-tRNA synthetase (AspRS), a component of the PURE cell-free transcription/translation system, in E. coli.
259942	pET28a-CK-Gg	Expression vector for producing chicken creatine kinase (CK), a component of the PURE cell-free transcription/translation system, in E. coli.
259943	pET28a-CysRS	Expression vector for producing E. coli cysteinyl-tRNA synthetase (CysRS), a component of the PURE cell-free transcription/translation system, in E. coli.
259944	pET28a-EFG	Expression vector for producing E. coli elongation factor G (EF-G), a component of the PURE cell-free transcription/translation system, in E. coli.
259945	pET28a-EFTs	Expression vector for producing E. coli elongation factor Ts (EF-Ts), a component of the PURE cell-free transcription/translation system, in E. coli.
259946	pET28a-EFTu	Expression vector for producing E. coli elongation factor Tu (EF-Tu), a component of the PURE cell-free transcription/translation system, in E. coli.
259947	pET28a-GlnRS	Expression vector for producing E. coli glutaminyl-tRNA synthetase (GlnRS), a component of the PURE cell-free transcription/translation system, in E. coli.
259948	pET28a-GluRS	Expression vector for producing E. coli glutamyl-tRNA synthetase (GluRS), a component of the PURE cell-free transcription/translation system, in E. coli.
259949	pET28a-GlyRS	Expression vector for producing E. coli glycyl-tRNA synthetase (GlyRS), a component of the PURE cell-free transcription/translation system, in E. coli.
259950	pET28a-HisRS	Expression vector for producing E. coli histidyl-tRNA synthetase (HisRS), a component of the PURE cell-free transcription/translation system, in E. coli.
259951	pET28a-IF1	Expression vector for producing E. coli translation initiation factor 1 (IF1), a component of the PURE cell-free transcription/translation system, in E. coli.
259953	pET28a-IleRS	Expression vector for producing E. coli isoleucyl-tRNA synthetase (IleRS), a component of the PURE cell-free transcription/translation system, in E. coli.
259954	pET28a-LeuRS	Expression vector for producing E. coli leucyl-tRNA synthetase (LeuRS), a component of the PURE cell-free transcription/translation system, in E. coli.
259955	pET28a-LysRS	Expression vector for producing E. coli lysyl-tRNA synthetase (LysRS), a component of the PURE cell-free transcription/translation system, in E. coli.
259956	pET28a-MTF	Expression vector for producing E. coli methionyl-tRNA transformylase, a component of the PURE cell-free transcription/translation system, in E. coli.
259957	pET28a-MetRS	Expression vector for producing E. coli methionyl-tRNA synthetase (MetRS), a component of the PURE cell-free transcription/translation system, in E. coli.
259958	pET28a-NDK	Expression vector for producing E. coli nucleoside diphosphate kinase (NDK), a component of the PURE cell-free transcription/translation system, in E. coli.
259959	pET28a-PPiase	Expression vector for producing E. coli PPiase, a component of the PURE cell-free transcription/translation system, in E. coli.
259960	pET28a-PheRS	Expression vector for producing E. coli phenylalanyl-tRNA synthetase (PheRS), a component of the PURE cell-free transcription/translation system, in E. coli.
259961	pET28a-ProRS	Expression vector for producing E. coli prolyl-tRNA synthetase (ProRS), a component of the PURE cell-free transcription/translation system, in E. coli.
259962	pET28a-RF1	Expression vector for producing E. coli release factor 1 (RF1), a component of the PURE cell-free transcription/translation system, in E. coli.
259963	pET28a-RF2	Expression vector for producing E. coli release factor 2 (RF2), a component of the PURE cell-free transcription/translation system, in E. coli.
259964	pET28a-RF3	Expression vector for producing E. coli release factor 3 (RF3), a component of the PURE cell-free transcription/translation system, in E. coli.
259965	pET28a-RRF	Expression vector for producing E. coli ribosome-releasing factor (RRF), a component of the PURE cell-free transcription/translation system, in E. coli.
259966	pET28a-SerRS	Expression vector for producing E. coli seryl-tRNA synthetase (SerRS), a component of the PURE cell-free transcription/translation system, in E. coli.
259967	pET28a-ThrRS	Expression vector for producing E. coli threonyl-tRNA synthetase (ThrRS), a component of the PURE cell-free transcription/translation system, in E. coli.
259968	pET28a-TrpRS	Expression vector for producing E. coli tryptophanyl-tRNA synthetase (TrpRS), a component of the PURE cell-free transcription/translation system, in E. coli.
259969	pET28a-TyrRS	Expression vector for producing E. coli tyrosyl-tRNA synthetase (TyrRS), a component of the PURE cell-free transcription/translation system, in E. coli.
259970	pET28a-ValRS	Expression vector for producing E. coli valyl-tRNA synthetase (ValRS), a component of the PURE cell-free transcription/translation system, in E. coli.
259971	pET28a-pT5-IF2	Expression vector for producing E. coli translation initiation factor 2 (IF2), a component of the PURE cell-free transcription/translation system, in E. coli.
259972	pET28a-pT5-T7RNAP	Expression vector for producing E. coli T7 RNA polymerase, a component of the PURE cell-free transcription/translation system, in E. coli.