A multi-species toolkit of TOP2 hypercleavage mutants for studying topoisomerase II-mediated DNA damage.

Ontoso D, Mehta M, Shabro A, Dittmar J, Reid RJD, Rothstein R, Nitiss JL, Keeney S
bioRxiv [Preprint]. 2026 May 11:2026.05.09.723998. doi: 10.64898/2026.05.09.723998. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
260230	pSK815 pRS415 pCUP1-yTOP2-F1025Y,R1128G-3xFLAG	yeast TOP2-F1025Y, R1128G with C-terminal 3xFLAG copper-inducible expression
260231	pSK955 pcDNA5-FRT-TO-hTOP2A-2xFLAG	human TOP2A WT with C-terminal 2xFLAG DOX-inducible expression