A multispecies toolkit of TOP2 hypercleavage mutants for studying topoisomerase II-mediated DNA damage.
Ontoso D, Mehta M, Shabro A, Dittmar J, Reid RJD, Rothstein R, Nitiss JL, Keeney S
Proc Natl Acad Sci U S A. 2026 Sep 8;123(36):e2616452123. doi: 10.1073/pnas.2616452123. Epub 2026 Sep 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 260230 | pSK815 pRS415 pCUP1-yTOP2-F1025Y,R1128G-3xFLAG | yeast TOP2-F1025Y, R1128G with C-terminal 3xFLAG copper-inducible expression |
| 260231 | pSK955 pcDNA5-FRT-TO-hTOP2A-2xFLAG | human TOP2A WT with C-terminal 2xFLAG DOX-inducible expression |