A genome-scale CRISPRi perturbation atlas of human induced pluripotent stem cells.
Nourreddine S, Doctor Y, Dailamy A, Lee YH, Hansen JN, Chinn R, Forget A, Polacco B, Muralidharan M, Sigaeva A, Sunder S, Pan E, Gao J, Chen JY, Clark T, Parker J, Obernier K, Metallo C, Ideker T, Lundberg E, Krogan N, Mali P
Nat Biotechnol. 2026 Jul 1. doi: 10.1038/s41587-026-03199-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 261262 | pPB-TRE3G-KRAB-dCas9-EGFP-HygR | PiggyBac vector to stably integrate doxycycline inducible KRAB(Zim3)-dCas9 |
| 262517 | pAAVS1-CAG-KRAB-dCas9-P2A-EGFP-BSD | CRISPRi cassette KRAB(Zim3)-dCas9 into the PPP1R12C locus AAVS1 safe-harbor locus. |
| 262518 | pAAVS1-BxB1-LP-mCherry-BSD | PPP1R12C/AAVS1 safe-harbor targeting donor for HDR that creates a BxB1 integrase landing pad. |