SMG6 interacts with the exon junction complex via two conserved EJC-binding motifs (EBMs) required for nonsense-mediated mRNA decay.
Kashima I, Jonas S, Jayachandran U, Buchwald G, Conti E, Lupas AN, Izaurralde E
Genes Dev. 2010 Nov 1. 24(21):2440-50. PubMed Article
Kashima I, Jonas S, Jayachandran U, Buchwald G, Conti E, Lupas AN, Izaurralde E
Genes Dev. 2010 Nov 1. 24(21):2440-50. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 146550 | pAc5.1B-V5-SBP-FLuc_I | Insect Expression of Fluc |
| 146551 | pCIneo-lambdaN-HA-HsSmg6_I | Mammalian Expression of HsSmg6 |
| 146552 | pCIneo-V5-SBP-HsSmg6_1-1238-siRNAres_I | Mammalian Expression of HsSmg6_1-1238-siRNAres |
| 146553 | pCIneo-V5-SBP-HsSmg6-del36-576-siRNAres_I | Mammalian Expression of HsSmg6-del36-576-siRNAres |
| 146554 | pCIneo-V5-SBP-HsSmg6-del577-816_I | Mammalian Expression of HsSmg6-del577-816 |
| 146555 | pCIneo-V5-SBP-HsSmg6-del817-1238-siRNAres_I | Mammalian Expression of HsSmg6-del817-1238-siRNAres |
| 146556 | pCIneo-V5-SBP-HsSmg6-siRNAres_I | Mammalian Expression of HsSmg6-siRNAres |
| 146557 | pEYFP-N1-HsSmg6_1-576_I | Mammalian Expression of HsSmg6_1-576 |
| 146558 | pEYFP-N1-HsSmg6_1-1238-siRNAres_I | Mammalian Expression of HsSmg6_1-1238-siRNAres |
| 146559 | pEYFP-N1-HsSmg6_577-816-siRNAres_I | Mammalian Expression of HsSmg6_577-816-siRNAres |
| 146560 | pEYFP-N1-HsSmg6_817-1238_I | Mammalian Expression of HsSmg6_817-1238-siRNAres |
| 146561 | pEYFP-N1-HsSmg6_1239-1419_I | Mammalian Expression of HsSmg6_1239-1419-siRNAres |
| 146562 | pEYFP-N1-HsSmg6-del36-576-siRNAres_I | Mammalian Expression of HsSmg6-del36-576-siRNAres |
| 146563 | pEYFP-N1-HsSmg6-del577-816_I | Mammalian Expression of HsSmg6-del577-816 |
| 146564 | pEYFP-N1-HsSmg6-del817-1238-siRNAres_I | Mammalian Expression of HsSmg6-del817-1238-siRNAres |
| 146565 | pEYFP-N1-HsSmg6-siRNAres_I | Mammalian Expression of HsSmg6-siRNAres |
| 146566 | pGEX-6P-1-HsSmg6_1-515_I | Bacterial Expression of HsSmg6_1-515 |
| 146567 | pLNHA-C1-HsUPF2-siRNAres_I | Mammalian Expression of HsUPF2-siRNAres |
| 146568 | pLNHA-C1-HsUPF3biso2_I | Mammalian Expression of HsUPF3Biso2 |
| 146569 | pN1-HsSmg6_1-576-V5-SBP-MBP_I | Mammalian Expression of HsSmg6_1-576 |
| 146570 | pN1-HsSmg6_577-816-siRNAres-V5-SBP-MBP_I | Mammalian Expression of HsSmg6_577-816-siRNAres |
| 146571 | pN1-HsSmg6_817-1238-V5-SBP-MBP_I | Mammalian Expression of HsSmg6_817-1238 |
| 146572 | pN1-HsSmg6_1239-1419-V5-SBP-MBP_I | Mammalian Expression of HsSmg6_1239-1419 |
| 146573 | pT7-EGFP-C1-HsUPF2-siRNAres_I | Mammalian Expression of HsUPF2-siRNAres |
| 146574 | pT7-EGFP-C1-HsUPF3biso2_I | Mammalian Expression of HsUPF3biso2 |
| 146748 | pCIneo-V5-SBP-HsSmg6-del42-58-del136-152-siRNAres_K | Mammalian Expression of HsSmg6-del42-58-del136-152-siRNAres |
| 146749 | pCIneo-V5-SBP-HsSmg6-del42-58-siRNAres_K | Mammalian Expression of HsSmg6-del42-58-siRNAres |
| 146750 | pCIneo-V5-SBP-HsSmg6-del136-152-siRNAres_K | Mammalian Expression of HsSmg6-del136-152-siRNAres |
| 146751 | pCIneo-V5-SBP-HsSmg6-EBM1-2mut-siRNAres_K | Mammalian Expression of HsSmg6-EBM1-2mut-siRNAres |
| 146752 | pCIneo-V5-SBP-HsSmg6-EBM1mut-siRNAres_K | Mammalian Expression of HsSmg6-EBM1mut-siRNAres |
| 146753 | pCIneo-V5-SBP-HsSmg6-EBM2mut-siRNAres_K | Mammalian Expression of HsSmg6-EBM2mut-siRNAres |
| 146754 | pETM41P-HsUPF3b-iso2_145-470_K | Bacterial Expression of HsUPF3biso2_145-470 |
| 146755 | pGEX-6P-1-HsSmg6_1-207_K | Bacterial Expression of HsSmg6_1-207 |
| 146756 | pGEX-6P-1-HsSmg6_1-207-EBM1-2mut_K | Bacterial Expression of HsSmg6_1-207-EBM1-2mut |
| 146757 | pGEX-6P-1-HsSmg6_1-207-EBM1mut_K | Bacterial Expression of HsSmg6_1-207-EBM1mut |
| 146758 | pGEX-6P-1-HsSmg6_1-207-EBM2mut_K | Bacterial Expression of HsSmg6_1-207-EBM2mut |
| 146759 | pGEX-6P-1-HsUPF3b-iso2_145-470_K | Bacterial Expression of HsUPF3biso2_145-470 |
| 146760 | pT7-EGFP-C1-HsC2orf68_K | Mammalian Expression of HsC2orf68 |
| 146761 | pT7-EGFP-C1-HsC2orf68-EBMmut_K | Mammalian Expression of HsC2orf68-EBMmut |
| 146762 | pT7-EGFP-C1-HsGIDRB86-EBMmut_K | Mammalian Expression of HsGIDRB86-EBMmut |
| 146763 | pT7-EGFP-C1-HsGIDRP86_K | Mammalian Expression of HsGIDRP86 |
| 146764 | pT7-EGFP-C1-HsR3HCC1_39-480_K | Mammalian Expression of HsR3HCC1_39-480 |
| 146765 | pT7-EGFP-C1-HsR3HCC1_39-480-EBMmut_K | Mammalian Expression of HsR3HCC1_39-480-EBMmut |
| 146766 | pT7-EGFP-C1-HsRRP12_K | Mammalian Expression of HsRRP12 |
| 146767 | pT7-EGFP-C1-HsRRP12-EBMmut_K | Mammalian Expression of HsRRP12-EBMmut |
| 146768 | pT7-EGFP-C1-HsTDRD3_K | Mammalian Expression of HsTDRD3 |
| 146769 | pT7-EGFP-C1-HsTDRD3-EBMmut_K | Mammalian Expression of HsTDRD3-EBMmut |