Rational design of a ligand-controlled protein conformational switch.
Dagliyan O, Shirvanyants D, Karginov AV, Ding F, Fee L, Chandrasekaran SN, Freisinger CM, Smolen GA, Huttenlocher A, Hahn KM, Dokholyan NV
Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6800-4. doi: 10.1073/pnas.1218319110. Epub 2013 Apr 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 45381 | pUSE-Src-YF-UniRapR-mCerulean-myc | Mammalian expression of constitutively active Src kinase (YF mutant) fused to uniRapR, which switches on activity in the presence of rapamycin. mCerulean for fluorescence visualization. |
| 45382 | pUSE-Src-KD-UniRapR-mCerulean-myc | Mammalian expression of kinase dead Src kinase (YF/KD mutant) fused to uniRapR. mCerulean for fluorescence visualization. |
| 45509 | Tol2 Krt4 Src (YF)-uniRapR-cerulean | Zebrafish transgenesis with constitutively active Src kinase (YF mutant) fused to uniRapR, which switches on activity in the presence of rapamycin. mCerulean for fluorescence visualization. |