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A triple helix stabilizes the 3' ends of long noncoding RNAs that lack poly(A) tails.
Wilusz JE, JnBaptiste CK, Lu LY, Kuhn CD, Joshua-Tor L, Sharp PA
Genes Dev. 2012 Nov 1;26(21):2392-407. doi: 10.1101/gad.204438.112. Epub 2012 Oct 16.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
46834pCRII-TOPO CMV-cGFP-mMALAT1_3' WT SenseExpress cGFP transcript ending in the wildtype MALAT1 triple helix
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46835pCRII-TOPO CMV-cGFP-bGH Poly(A) SenseExpress cGFP transcript ending in a poly(A) tail [using the bGH poly(A) signal]
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46836pCRII-TOPO CMV-cGFP-SV40 Poly(A) SenseExpress cGFP transcript ending in a poly(A) tail [using the SV40 poly(A) signal]
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46837pCRII-TOPO CMV-cGFP-mMALAT1_3' Mut U1/U2 SenseExpress cGFP transcript ending in a mutant MALAT1 triple helix which causes the transcript to be degraded
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46838pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.14 SenseExpress cGFP transcript ending in the minimal functional MALAT1 triple helix
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46839pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 SenseExpress cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translated
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46840pCRII-TOPO CMV-cGFP-mMEN b_3' SenseExpress cGFP transcript ending in the wildtype MEN beta triple helix
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