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Efficient site-specific transgenesis and enhancer activity tests in medaka using PhiC31 integrase.
Kirchmaier S, Hockendorf B, Moller EK, Bornhorst D, Spitz F, Wittbrodt J
Development. 2013 Sep 18.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
48875pSB_PhiC31LandingSiteSleeping beauty transposon with the PhiC31 landing site in a cmlc2::EGFP selection cassette
48876pEx_MCS-attBtagRFPtPhiC31 exchange vector with multiple cloning site, exchange of EGFP in landing site with tagRFPt
48877pEx_Hsp70::EGFPpA-ttBtagRFPtPhiC31 exchange vector with the Hsp70::EGFPpA insert. Selection via EGFP to tagRFPt-color switch in the heart
48878pEx_Hsp70::attBmutPhiC31 exchange vector with the Hsp70 promoter driving tagRFPt from the landing site. Template for enhancer tests.

Antibodies from Article