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Biff Forbush Lab: Forbush Lab human NKCC1 NT clones
Unpublished

Plasmids from Article

ID Plasmid Purpose  
49062pcDNA3.1 Flag YFP hNKCC1 N-terminal deletion (NT53)Expresses human NKCC1 truncation mutant (aa13-222) with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50871pcDNA3.1 Flag YFP hNKCC1 E670C (NT473)Expresses human NKCC1 E670C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50872pcDNA3.1 Flag YFP hNKCC1 L671C (NT440)Expresses human NKCC1 L671C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50882pcDNA3.1 Flag YFP hNKCC1 F728C (NT502)Expresses human NKCC1 F728C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50883pcDNA3.1 Flag YFP hNKCC1 V729C (NT503)Expresses human NKCC1 V729C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50886pcDNA3.1 Flag YFP hNKCC1 W732C (NT506)Expresses human NKCC1 W732C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50887pcDNA3.1 Flag YFP hNKCC1 W733C (NT507)Expresses human NKCC1 W733C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50888pcDNA3.1 Flag YFP hNKCC1 A734C (NT508)Expresses human NKCC1 A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50892pcDNA3.1 Flag YFP hNKCC1 T738C (NT524)Expresses human NKCC1 T738C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50893pcDNA3.1 Flag YFP hNKCC1 Y739C (NT525)Expresses human NKCC1 Y739C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50894pcDNA3.1 Flag YFP hNKCC1 V740C (NT526)Expresses human NKCC1 V740C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50895pcDNA3.1 Flag YFP hNKCC1 S716C C723S C724V (NT530)Expresses human NKCC1 S716C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50896pcDNA3.1 Flag YFP hNKCC1 L717C C723S C724V (NT531)Expresses human NKCC1 L717C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50897pcDNA3.1 Flag YFP hNKCC1 L718C C723S C724V (NT532)Expresses human NKCC1 L718C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50898pcDNA3.1 Flag YFP hNKCC1 G719C C723S C724V (NT533)Expresses human NKCC1 G719C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50899pcDNA3.1 Flag YFP hNKCC1 A720C C723S C724V (NT534)Expresses human NKCC1 A720C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50900pcDNA3.1 Flag YFP hNKCC1 I721C C723S C724V (NT535)Expresses human NKCC1 I721C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50901pcDNA3.1 Flag YFP hNKCC1 L722C C723S C724V (NT536)Expresses human NKCC1 L722C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50902pcDNA3.1 Flag YFP hNKCC1 C723S (NT537)Expresses human NKCC1 C723S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50903pcDNA3.1 Flag YFP hNKCC1 C723S C724V I725C (NT538)Expresses human NKCC1 C723S C724V I725C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50904pcDNA3.1 Flag YFP hNKCC1 C723S C724V V726C (NT539)Expresses human NKCC1 C723S C724V V726C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50908pcDNA3.1 Flag YFP hNKCC1 C723S C724V W732C (NT546)Expresses human NKCC1 C723S C724V W732C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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50911pcDNA3.1 Flag YFP hNKCC1 V729S (NT553)Expresses human NKCC1 V729S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
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