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Molecular Motions Involved in Na-K-Cl Cotransporter-mediated Ion Transport and Transporter Activation Revealed by Internal Crosslinking between Transmembrane Domains 10 and 11/12.
Monette MY, Somasekharan S, Forbush B
J Biol Chem. 2014 Jan 22. PubMed Article

Plasmids from Article

ID Plasmid Purpose
50863pcDNA3.1 Flag YFP hNKCC1 I674C I730C (NT835)Expresses human NKCC1 I674C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50864pcDNA3.1 Flag YFP hNKCC1 A675C A734C (NT841)Expresses human NKCC1 A675C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50865pcDNA3.1 Flag YFP hNKCC1 A675C C723S C724V A735C (NT847)Expresses human NKCC1 A675C C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50866pcDNA3.1 Flag YFP hNKCC1 P676C I730C (NT809)Expresses human NKCC1 P676C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50867pcDNA3.1 Flag YFP hNKCC1 P676C A734C (NT810)Expresses human NKCC1 P676C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50868pcDNA3.1 Flag YFP hNKCC1 I677C A734C (NT813)Expresses human NKCC1 I677C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50869pcDNA3.1 Flag YFP hNKCC1 S679C I730C (NT838)Expresses human NKCC1 S679C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50870pcDNA3.1 Flag YFP hNKCC1 N680C A734C (NT816)Expresses human NKCC1 N680C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50873pcDNA3.1 Flag YFP hNKCC1 N672C (NT474)Expresses human NKCC1 N672C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50874pcDNA3.1 Flag YFP hNKCC1 V673C (NT437)Expresses human NKCC1 V673C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50875pcDNA3.1 Flag YFP hNKCC1 I674C (NT475)Expresses human NKCC1 I674C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50876pcDNA3.1 Flag YFP hNKCC1 A675C (NT441)Expresses human NKCC1 A675C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50877pcDNA3.1 Flag YFP hNKCC1 P676C (NT443)Expresses human NKCC1 P676C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50878pcDNA3.1 Flag YFP hNKCC1 I677C (NT467)Expresses human NKCC1 I677C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50879pcDNA3.1 Flag YFP hNKCC1 I678C (NT445)Expresses human NKCC1 I678C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50880pcDNA3.1 Flag YFP hNKCC1 S679C (NT447)Expresses human NKCC1 S679C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50881pcDNA3.1 Flag YFP hNKCC1 N680C (NT468)Expresses human NKCC1 N680C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50884pcDNA3.1 Flag YFP hNKCC1 I730C (NT504)Expresses human NKCC1 I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50885pcDNA3.1 Flag YFP hNKCC1 N731C (NT505)Expresses human NKCC1 N731C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50889pcDNA3.1 Flag YFP hNKCC1 A735C (NT509)Expresses human NKCC1 A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50890pcDNA3.1 Flag YFP hNKCC1 L736C (NT522)Expresses human NKCC1 L736C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50891pcDNA3.1 Flag YFP hNKCC1 L737C (NT523)Expresses human NKCC1 L737C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50905pcDNA3.1 Flag YFP hNKCC1 C723S C724V M727C (NT540)Expresses human NKCC1 C723S C724V M727C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50906pcDNA3.1 Flag YFP hNKCC1 C723S C724V F728C (NT542)Expresses human NKCC1 C723S C724V F728C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50907pcDNA3.1 Flag YFP hNKCC1 C723S C724V V729C (NT543)Expresses human NKCC1 C723S C724V V729C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50909pcDNA3.1 Flag YFP hNKCC1 C723S C724V W733C (NT547)Expresses human NKCC1 C723S C724V W733C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50910pcDNA3.1 Flag YFP hNKCC1 C723S C724V A735C (NT549)Expresses human NKCC1 C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
50912pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion C-terminus replaced NBCe C-terminus (NT94)Expresses human NKCC1 with truncation of N-terminus, mutations at P676C I730C and C-terminus replaced with that of NBCe. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells
50913pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations P676C I730C (NT95)Expresses human NKCC1 with mutated Ser and Thr residues in the N-terminus and mutations at P676C I730C. Contains N-terminal 3xFLAG-YFP tag for expression in mammalian cells
50914pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion (NT96)Expresses human NKCC1 with truncation of N-terminus and mutations at P676C I730C. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells

Antibodies from Article