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Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases.
Zhuang M, Calabrese MF, Liu J, Waddell MB, Nourse A, Hammel M, Miller DJ, Walden H, Duda DM, Seyedin SN, Hoggard T, Harper JW, White KP, Schulman BA
Mol Cell. 2009 Oct 9;36(1):39-50. doi: 10.1016/j.molcel.2009.09.022.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
52293Split 'n coexpress CUL3-RBX1bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1
52294His-MBP human SPOP (aa 28-337)bacterial expression of human SPOP (aa 28-337) fused to His-MBP
52295His Puc (aa 1-393)Bacterial expression of His tagged Drosophila Puc fragment

Antibodies from Article