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CHES1/FOXN3 regulates cell proliferation by repressing PIM2 and protein biosynthesis.
Huot G, Vernier M, Bourdeau V, Doucet L, Saint-Germain E, Gaumont-Leclerc MF, Moro A, Ferbeyre G
Mol Biol Cell. 2014 Mar;25(5):554-65. doi: 10.1091/mbc.E13-02-0110. Epub 2014 Jan 8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
51836pLPC-Flag-CHES1Lencodes the long isoform of human CHES1 and a Flag tag
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51837pLPC-Flag-CHES11L-dFCFlag-CHES1L mutant lacking the forkhead binding site and the C-terminal domains
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51838pLPC-Flag-CHES1LdFC-NLSFlag-CHES1L mutant lacking the forkhead binding site and C-terminal domain, with remaining NLS
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51839pLPC-Flag-CHES1L-dCFlag-CHES1L mutant lacking the C-terminal and NLS domains
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51840pLPC-Flag-CHES1L-dC-NLSFlag-CHES1L mutant lacking the C-terminal, with remaining NLS
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51841pLPC-Flag-CHES1L-DBD-NLSFlag-CHES1L mutant encoding the DNA binding and NLS domains only
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51842pLPC-Flag-CHES1L-dFNFlag-CHES1L mutant lacking the forkhead binding site and N-terminal domains
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51843pLPC-Flag-CHES1L-dNFlag-CHES1L mutant lacking the N-terminal domain
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51844pLPC-Flag-CHES1H164AFlag-CHES1L mutant with ponctual mutation on histidine 164
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51845mlp-hCHES1shshRNA against CHES1
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51846pBabe-CHES1-ERfusion protein between CHES1 and the ligand binding domain of estrogen receptor
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51847pWZL-rCHES1encodes a CHES1 mRNA that is resistant to shRNA against CHES1
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51848pGL3-PIM2promoter region (-550;0) of PIM2
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51849pGL3-PIM2mut1promoter region of PIM2 lacking the forkhead binding site #2
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51850pGL3-PIM2mut2promoter region of PIM2 lacking the forkhead binding site #1
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53742pGL3-PIM2mut3promoter region of PIM2 lacking the 2 forkhead binding sites
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53743pLPC-CHES1Llong isoform of human CHES1
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Antibodies from Article