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S-palmitoylation and ubiquitination differentially regulate interferon-induced transmembrane protein 3 (IFITM3)-mediated resistance to influenza virus.
Yount JS, Karssemeijer RA, Hang HC
J Biol Chem. 2012 Jun 1;287(23):19631-41. doi: 10.1074/jbc.M112.362095. Epub 2012 Apr 17.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
58400pCMV-HA-mIFITM3-K24AExpresses murine IFITM3-K24A with an N-terminal HA tag in mammalian cells
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58401pCMV-HA-mIFITM3-K83AExpresses murine IFITM3-K83A with an N-terminal HA tag in mammalian cells
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58402pCMV-HA-mIFITM3-K88AExpresses murine IFITM3-K88A with an N-terminal HA tag in mammalian cells
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58403pCMV-HA-mIFITM3-K104AExpresses murine IFITM3-K104A with an N-terminal HA tag in mammalian cells
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58404pCMV-HA-mIFITM3-K24,83,88AExpresses murine IFITM3-K24,83,88A with an N-terminal HA tag in mammalian cells
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58405pCMV-HA-mIFITM3-K83,88,104AExpresses murine IFITM3-K83,88,104A with an N-terminal HA tag in mammalian cells
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58406pCMV-HA-mIFITM3-K24,88,104AExpresses murine IFITM3-K24,88,104A with an N-terminal HA tag in mammalian cells
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58407pCMV-HA-mIFITM3-K24,83,104AExpresses murine IFITM3-K24,83,104A with an N-terminal HA tag in mammalian cells
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58408pCMV-HA-mIFITM3-K24,83,88,104AExpresses murine IFITM3-K24,83,88,104A with an N-terminal HA tag in mammalian cells (Lysine-less nonubiquitinated mutant)
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58410pCMV-HA-mIFITM3-N2AExpresses murine IFITM3-N2A with an N-terminal HA tag in mammalian cells (mutates possible glycosylation site)
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58411pCMV-HA-mIFITM3-S38NExpresses murine IFITM3-S38N with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)
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58412pCMV-HA-mIFITM3-D92NExpresses murine IFITM3-D92N with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)
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58413pCMV-HA-mIFITM3-H136TExpresses murine IFITM3-H136T with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)
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58414pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVLExpresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cells
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Antibodies from Article