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Palmitoylation on conserved and nonconserved cysteines of murine IFITM1 regulates its stability and anti-influenza A virus activity.
Hach JC, McMichael T, Chesarino NM, Yount JS
J Virol. 2013 Sep;87(17):9923-7. doi: 10.1128/JVI.00621-13. Epub 2013 Jun 26. PubMed Article

Plasmids from Article

ID Plasmid Purpose
58415pCMV-HA-mIFITM1Expresses murine IFITM1 with an N-terminal HA tag in mammalian cells.
58416pCMV-HA-mIFITM1-C49AExpresses murine IFITM1-C49A with an N-terminal HA tag in mammalian cells
58417pCMV-HA-mIFITM1-C50AExpresses murine IFITM1-C50A with an N-terminal HA tag in mammalian cells.
58418pCMV-HA-mIFITM1-C83AExpresses murine IFITM1-C83A with an N-terminal HA tag in mammalian cells.
58419pCMV-HA-mIFITM1-C103AExpresses murine IFITM1-C103A with an N-terminal HA tag in mammalian cells.
58420pCMV-HA-mIFITM1-C49,50,83AExpresses murine IFITM1-C49,50,83A with an N-terminal HA tag in mammalian cells. (mutated at the 3 evolutionarily conserved cysteines)
58421pCMV-HA-mIFITM1-C49,50,83,103AExpresses murine IFITM1-C49,50,83,103A with an N-terminal HA tag in mammalian cells (cysteine-less mutant that is not palmitoylated).

Antibodies from Article