UNIGEMS: plasmids and parts to facilitate teaching on assembly, gene expression control and logic in E. coli
Siddall A, Williams AA, Sanders J, Denton JA, Madden D, Schollar J, Bryk J
bioRxiv 2021.06.20.449138 Article
Siddall A, Williams AA, Sanders J, Denton JA, Madden D, Schollar J, Bryk J
bioRxiv 2021.06.20.449138 Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 58533 | p005kanRFP | RFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant. |
| 58534 | p006kanGFP | GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant. |
| 58535 | p007ampGFP | GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; ampicillin resistant. |
| 108313 | p006-GFP-strong | GFP from jellyfish Aquorea sp. under strong synthetic constitutive promoter pFAB4026; kanamycin resistant. |
| 108314 | p006-GFP-weak | GFP from jellyfish Aquorea sp. under weak synthetic constitutive promoter pFAB4282; kanamycin resistant. |
| 108315 | p006-GFP-pBAD | GFP from jellyfish Aquorea sp. under pBAD promoter with AraC repressor; kanamycin resistant. |
| 108316 | p005-RFP-weak | Synthetic RFP based on Corynactis spp under weak synthetic constitutive promoter pFAB4024; kanamycin resistant. |
| 108317 | p005-RFP-strong | Synthetic RFP based on Corynactis spp under strong synthetic constitutive promoter pFAB4005; kanamycin resistant. |
| 112237 | p006-GFP-Logic-AND | GFP from jellyfish Aquorea sp. under D61 AND gate-like ara+lac promoter with AraC repressor; lacI repressor; kanamycin resistant. |
| 112251 | p006-Banana-Late | ATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant. |