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A 2in1 cloning system enables ratiometric bimolecular fluorescence complementation (rBiFC).
Grefen C, Blatt MR
Biotechniques. 2012 Oct 2;53(5):311-14. doi: 10.2144/000113941. PubMed Article

Plasmids from Article

ID Plasmid Purpose
105109pUC57-R3R2 create your own 2in1 constructs
105110pUC57-R1R4create your own 2in1 constructs
105111pBiFCt-2in1-NNratiometric BiFC analysis in plants (transient transformation)
105112pBiFCt-2in1-NCratiometric BiFC analysis in plants (transient transformation)
105113pBiFCt-2in1-CNratiometric BiFC analysis in plants (transient transformation)
105114pBiFCt-2in1-CCratiometric BiFC analysis in plants (transient transformation)

Antibodies from Article