SOX17 is a critical specifier of human primordial germ cell fate.
Irie N, Weinberger L, Tang WW, Kobayashi T, Viukov S, Manor YS, Dietmann S, Hanna JH, Surani MA
Cell. 2015 Jan 15;160(1-2):253-68. doi: 10.1016/j.cell.2014.12.013. Epub 2014 Dec 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 59721 | Human NANOS3-P2A-mCherry knock-in targeting construct | Gene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised. |
| 59722 | FOR human NANOS3 TALEN | TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721) |
| 59723 | REV human NANOS3 TALEN | TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721) |
| 59724 | Human BLIMP1 sgRNA | Plasmid encoding sgRNA to generate BLIMP1 knockout mutant human cells |
| 59725 | Human SOX17 sgRNA | Plasmid encoding sgRNA to generate SOX17 knock-out mutant human cells |
| 59726 | Human T (BRACHYURY) sgRNA | Plasmid encoding sgRNA to generate T (BRACHYURY) knock-out mutant human cells |