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SOX17 Is a Critical Specifier of Human Primordial Germ Cell Fate
Naoko Irie, Leehee Weinberger, Walfred W.C. Tang, Toshihiro Kobayashi, Sergey Viukov, Yair S. Manor, Sabine Dietmann, Jacob H. Hanna, M. Azim Surani
Cell 160, 1–16, January 15, 2015
Article

Plasmids from Article

ID Plasmid Purpose  
59721Human NANOS3-P2A-mCherry knock-in targeting constructGene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.
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59722FOR human NANOS3 TALENTALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)
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59723REV human NANOS3 TALENTALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)
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59724Human BLIMP1 sgRNAPlasmid encoding sgRNA to generate BLIMP1 knockout mutant human cells
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59725Human SOX17 sgRNAPlasmid encoding sgRNA to generate SOX17 knock-out mutant human cells
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59726Human T (BRACHYURY) sgRNAPlasmid encoding sgRNA to generate T (BRACHYURY) knock-out mutant human cells
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