REV human NANOS3 TALEN
PurposeTALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||59723||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepc-Goldy TALEN
Backbone manufacturerDan Carlson lab plasmids (from addgene)
Vector typeMammalian Expression, TALEN
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameNG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI
- Promoter CAGGS
- Cloning method Restriction Enzyme
- 5′ cloning site Esp3I (destroyed during cloning)
- 3′ cloning site Esp3I (destroyed during cloning)
- 5′ sequencing primer AGGCGACACACGAAGACATC
- 3′ sequencing primer CGGCGATTGACTCTTCTGAT (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:REV human NANOS3 TALEN was a gift from Jacob Hanna (Addgene plasmid # 59723 ; http://n2t.net/addgene:59723 ; RRID:Addgene_59723)
For your References section:SOX17 Is a Critical Specifier of Human Primordial Germ Cell Fate. Naoko Irie, Leehee Weinberger, Walfred W.C. Tang, Toshihiro Kobayashi, Sergey Viukov, Yair S. Manor, Sabine Dietmann, Jacob H. Hanna, M. Azim Surani. Cell 160, 1–16, January 15, 2015 10.1016/j.cell.2014.12.013