Biased Brownian ratcheting leads to pre-mRNA remodeling and capture prior to first-step splicing.
Krishnan R, Blanco MR, Kahlscheuer ML, Abelson J, Guthrie C, Walter NG
Nat Struct Mol Biol. 2013 Dec;20(12):1450-7. doi: 10.1038/nsmb.2704. Epub 2013 Nov 17. PubMed Article
Krishnan R, Blanco MR, Kahlscheuer ML, Abelson J, Guthrie C, Walter NG
Nat Struct Mol Biol. 2013 Dec;20(12):1450-7. doi: 10.1038/nsmb.2704. Epub 2013 Nov 17. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 61042 | Prp2-pRSETA | Encodes the yeast Prp2 protein for overexpression and purification using a C-terminal hexahistidine tag. |
| 61043 | Cwc25-pRSETA | Encodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling. |