Computational design of a red fluorophore ligase for site-specific protein labeling in living cells.
Liu DS, Nivon LG, Richter F, Goldman PJ, Deerinck TJ, Yao JZ, Richardson D, Phipps WS, Ye AZ, Ellisman MH, Drennan CL, Baker D, Ting AY
Proc Natl Acad Sci U S A. 2014 Oct 28;111(43):E4551-9. doi: 10.1073/pnas.1404736111. Epub 2014 Oct 13. PubMed Article
Liu DS, Nivon LG, Richter F, Goldman PJ, Deerinck TJ, Yao JZ, Richardson D, Phipps WS, Ye AZ, Ellisman MH, Drennan CL, Baker D, Ting AY
Proc Natl Acad Sci U S A. 2014 Oct 28;111(43):E4551-9. doi: 10.1073/pnas.1404736111. Epub 2014 Oct 13. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 61307 | pcDNA3-FLAG-LplA(AAG) | Contains FLAG epitope for immunofluorescence detection of resorufin ligase for mammalian cell expression |
| 61820 | pcDNA3-GFP-LplA(AAG) | Contains GFP tag for direct live-cell fluorescence imaging of the resofurin ligase |
| 61821 | pcDNA3-GFP-LpIA(wild-type) | Encodes a wild-type sequence of LplA and serves as the negative control for resorufin labeling |