Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity.
Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, Liu DR
Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 2014 Feb 16. PubMed Article
Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, Liu DR
Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 2014 Feb 16. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 51438 | TALEN-CCR5L18-CtermCan | Expresses TALEN targeting left CCR5A site (L18) with canonical C-terminal domain |
| 51439 | TALEN-CCR5R18-CtermCan | Expresses TALEN targeting right CCR5A site (R18) with canonical C-terminal domain |
| 51440 | TALEN-CCR5L18-CtermQ3 | Expresses TALEN targeting left CCR5A site (L18) with Q3 C-terminal domain |
| 51441 | TALEN-CCR5R18-CtermQ3 | Expresses TALEN targeting right CCR5A site (R18) with Q3 C-terminal domain |
| 51442 | TALEN-CCR5L18-CtermQ7 | Expresses TALEN targeting left CCR5A site (L18) with Q7 C-terminal domain |
| 51443 | TALEN-CCR5R18-CtermQ7 | Expresses TALEN targeting right CCR5A site (R18) with Q7 C-terminal domain |
| 51444 | TALEN-CCR5L18-NtermN1 | Expresses TALEN targeting left CCR5A site (L18) with N1 N-terminal domain |
| 51445 | TALEN-CCR5R18-NtermN1 | Expresses TALEN targeting right CCR5A site (R18) with N1 N-terminal domain |
| 51446 | TALEN-CCR5L18-NtermN2 | Expresses TALEN targeting left CCR5A site (L18) with N2 N-terminal domain |
| 51447 | TALEN-CCR5R18-NtermN2 | Expresses TALEN targeting right CCR5A site (R18) with N2 N-terminal domain |
| 51448 | TALEN-CCR5L18-NtermN3 | Expresses TALEN targeting left CCR5A site (L18) with N3 N-terminal domain |
| 51449 | TALEN-CCR5R18-NtermN3 | Expresses TALEN targeting right CCR5A site (R18) with N3 N-terminal domain |