Escherichia coli auxotroph host strains for amino acid-selective isotope labeling of recombinant proteins.
Lin MT, Fukazawa R, Miyajima-Nakano Y, Matsushita S, Choi SK, Iwasaki T, Gennis RB
Methods Enzymol. 2015;565:45-66. doi: 10.1016/bs.mie.2015.05.012. Epub 2015 Jun 10. PubMed Article
Lin MT, Fukazawa R, Miyajima-Nakano Y, Matsushita S, Choi SK, Iwasaki T, Gennis RB
Methods Enzymol. 2015;565:45-66. doi: 10.1016/bs.mie.2015.05.012. Epub 2015 Jun 10. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 61953 | YM138 | C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. cysE gene deleted. |
| 61960 | MS1 | C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. cysE hisG genes deleted |
| 61961 | RF11 | C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. metA gene deleted |
| 61962 | RF1 | BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. glyA gene deleted |
| 62070 | RF2 | BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC gene deleted |
| 62072 | RF4 | BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB genes deleted. |
| 62073 | RF5 | BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB hisG genes deleted. |
| 62074 | RF6 | BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. proC gene deleted. |
| 62075 | RF8 | BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. asnA asnB genes deleted. |
| 62076 | RF10 | BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. lysA genes deleted. |
| 62077 | RF12 | BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. trpA trpB genes deleted. |
| 62078 | RF13 | BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB genes deleted. |